Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns
المؤلفون المشاركون
Zivny, J.
Vítková, Veronika
Pánek, Martin
Janec, Petr
Šibíková, Michaela
Vobruba, Václav
Haluzík, Martin
Janota, Jan
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-07-19
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Neonatal systemic inflammatory response and multiple organ dysfunction syndrome are the main postnatal insults influencing mortality and morbidity.
Critically ill newborns with high predicted mortality are supported by extracorporeal membrane oxygenation (ECMO).
Biomarkers of inflammatory response and endothelial injury can be used for early diagnosis and treatment of critical neonatal situations.
The aim of our study was to explore plasma proteins and endothelial microvesicles as markers of inflammation and endothelial activation in newborns on ECMO and to compare them with healthy neonates.
Thirteen newborns on ECMO and 13 healthy newborns were included in the study.
Plasma soluble biomarkers were measured using multiplex immunoassay based on Luminex® xMAP multianalyte profiling platform.
The total microvesicle count and plasma level of surface antigen-specific microvesicles were determined by flow cytometry.
The plasma concentration of cell-derived microvesicles was measured using annexin-V labeling, and the endothelial origin of microvesicles was determined using lineage-specific antigen labeling of endothelial cell/microvesicle markers (endoglin/CD105, PECAM1/CD31, VEGFR2/CD309, and MadCAM1).
Inflammatory markers (procalcitonin, IL-1β, IL-6, and IL-22) were increased in the ECMO group (P<0.01).
The assessment of endothelial markers showed higher concentrations of endocan and angiopoietin-2 (P<0.01) in the ECMO group while VEGF in the ECMO group was significantly lower (P<0.01).
In the ECMO group, the concentration of annexin-V-positive microvesicles (total microvesicles) and endothelial microvesicles expressing mucosal vascular addressin cell adhesion molecule 1 (MadCAM1) was increased (P=0.05).
In summary, we found increased concentrations of soluble inflammatory and endothelial markers in the plasma of critically ill newborns with multiple organ dysfunction.
Increased plasma concentrations of microvesicles may reflect the activation or damage of blood cells and vasculature including endothelial cells.
The measurement of cell membrane-derived microvesicles may be added to the panel of established inflammatory markers in order to increase the sensitivity and specificity of the diagnostic process in critically ill newborns.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Vítková, Veronika& Pánek, Martin& Janec, Petr& Šibíková, Michaela& Vobruba, Václav& Haluzík, Martin…[et al.]. 2018. Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1203413
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Vítková, Veronika…[et al.]. Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns. Mediators of Inflammation No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1203413
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Vítková, Veronika& Pánek, Martin& Janec, Petr& Šibíková, Michaela& Vobruba, Václav& Haluzík, Martin…[et al.]. Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1203413
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1203413
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر