Alpha-Synuclein Preserves Mitochondrial Fusion and Function in Neuronal Cells
المؤلفون المشاركون
Longhena, Francesca
Bellucci, Arianna
Faustini, Gaia
Marchesan, Elena
Zonta, Laura
Bono, Federica
Bottani, Emanuela
Ziviani, Elena
Valerio, Alessandra
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-23
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Dysregulations of mitochondria with alterations in trafficking and morphology of these organelles have been related to Parkinson’s disease (PD), a neurodegenerative disorder characterized by brain accumulation of Lewy bodies (LB), intraneuronal inclusions mainly composed of α-synuclein (α-syn) fibrils.
Experimental evidence supports that α-syn pathological aggregation can negatively impinge on mitochondrial functions suggesting that this protein may be crucially involved in the control of mitochondrial homeostasis.
The aim of this study was to assay this hypothesis by analyzing mitochondrial function and morphology in primary cortical neurons from C57BL/6JOlaHsd α-syn null and C57BL/6J wild-type (wt) mice.
Primary cortical neurons from mice lacking α-syn showed decreased respiration capacity measured with a Seahorse XFe24 Extracellular Flux Analyzer.
In addition, morphological Airyscan superresolution microscopy showed the presence of fragmented mitochondria while real-time PCR and western blot confirmed altered expression of proteins involved in mitochondrial shape modifications in the primary cortical neurons of α-syn null mice.
Transmission electron microscopy (TEM) studies showed that α-syn null neurons exhibited impaired mitochondria-endoplasmic reticulum (ER) physical interaction.
Specifically, we identified a decreased number of mitochondria-ER contacts (MERCs) paralleled by a significant increase in ER-mitochondria distance (i.e., MERC length).
These findings support that α-syn physiologically preserves mitochondrial functions and homeostasis.
Studying α-syn/mitochondria interplay in health and disease is thus pivotal for understanding their involvement in PD and other LB disorders.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Faustini, Gaia& Marchesan, Elena& Zonta, Laura& Bono, Federica& Bottani, Emanuela& Longhena, Francesca…[et al.]. 2019. Alpha-Synuclein Preserves Mitochondrial Fusion and Function in Neuronal Cells. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1203527
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Faustini, Gaia…[et al.]. Alpha-Synuclein Preserves Mitochondrial Fusion and Function in Neuronal Cells. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1203527
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Faustini, Gaia& Marchesan, Elena& Zonta, Laura& Bono, Federica& Bottani, Emanuela& Longhena, Francesca…[et al.]. Alpha-Synuclein Preserves Mitochondrial Fusion and Function in Neuronal Cells. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1203527
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1203527
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر