Grape Seed Proanthocyanidin Extract Inhibits Human Esophageal Squamous Cancerous Cell Line ECA109 via the NF-κB Signaling Pathway
المؤلفون المشاركون
Li, Shugang
Ding, Yu-Song
Ma, Ru-Lin
Niu, Qiang
Hu, Yun-Hua
Xie, Jianxin
Guo, Fangming
Li, Yu
Wang, Xianhua
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-12-17
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Esophageal squamous cell carcinoma is the most common type of squamous cell carcinoma.
Grape seed proanthocyanidin extract (GSPE) is considered to exhibit anticancer activity against several different types of cancer.
We aimed to determine whether GSPE inhibited esophageal squamous cancerous cells and the possible involvement of NF-κB in this process.
The human esophageal squamous cancer cell line ECA109 was treated with GSPE (0–80 μg/mL) and BAY11-7082 (10 μmol/L) for 12, 24, and 48 h.
The MTT assay was used to determine cell proliferation; alterations in cell apoptosis were detected by flow cytometry; levels of inflammatory factors interleukin-6 and cyclooxygenase-2 and apoptotic proteins Bax/Bcl-2 were measured by ELISA; qRT-PCR and western blots were used to examine the activation of caspase-3 and NF-κB signaling.
GSPE inhibited the proliferation of ECA109 cells and induced cellular apoptosis in a time- and dose-dependent manner.
ELISA results showed that GSPE and BAY11-7082 reduced the secretion of inflammatory cytokines interleukin-6 and cyclooxygenase-2.
The results of PCR and western blotting indicated that GSPE and BAY11-7082 activated caspase-3 and attenuated the activation of the NF-κB signaling pathway.
GSPE induced apoptosis in ECA109 cells and inhibited ECA109 cell proliferation via a reduction in the secretion of inflammatory cytokines.
This mechanism may be related to the attenuation of NF-κB activity and the sensitization of caspase-3.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Guo, Fangming& Hu, Yun-Hua& Niu, Qiang& Li, Yu& Ding, Yu-Song& Ma, Ru-Lin…[et al.]. 2018. Grape Seed Proanthocyanidin Extract Inhibits Human Esophageal Squamous Cancerous Cell Line ECA109 via the NF-κB Signaling Pathway. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1203556
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Guo, Fangming…[et al.]. Grape Seed Proanthocyanidin Extract Inhibits Human Esophageal Squamous Cancerous Cell Line ECA109 via the NF-κB Signaling Pathway. Mediators of Inflammation No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1203556
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Guo, Fangming& Hu, Yun-Hua& Niu, Qiang& Li, Yu& Ding, Yu-Song& Ma, Ru-Lin…[et al.]. Grape Seed Proanthocyanidin Extract Inhibits Human Esophageal Squamous Cancerous Cell Line ECA109 via the NF-κB Signaling Pathway. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1203556
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1203556
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر