Evaluation of Binding of Rosmarinic Acid with Human Transferrin and Its Impact on the Protein Structure: Targeting Polyphenolic Acid-Induced Protection of Neurodegenerative Disorders
المؤلفون المشاركون
Al-Ajmi, Mohamed F.
Shahbaaz, Mohd
Hussain, Afzal
Shamsi, Anas
Anwar, Saleha
Mohammad, Taj
Hassan, Imtaiyaz
Ahmad, Faizan
Islam, Asimul
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-05
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Rosmarinic acid (RA) is a natural compound that is gaining wide popularity owing to its broad-spectrum biological activities.
RA is known for its wide range of medicinal properties and therapeutic applications in a vast range of neurodegenerative disorders thus making it a vital natural compound.
Human transferrin (hTf) is a clinically significant protein that plays a pivotal role in maintaining iron homeostasis.
The importance of studies pertaining to hTf is attributable to the pivotal role of iron deposition in CNS in neurodegenerative disorders.
The study was intended to have an insight into the interaction between RA and hTf employing multispectroscopic approach, molecular docking, and molecular dynamic simulation studies.
Fluorescence quenching studies revealed that RA shows an excellent binding affinity to hTf with a binding constant (K) of 107 M-1 and is guided by static mode of quenching.
Isothermal titration calorimetry (ITC) further validated the spontaneous nature of binding.
The estimation of enthalpy change (∆H) and entropy change (∆S) suggested that the RA-hTf complex formation is driven by hydrogen bonding, thereby making this process seemingly specific.
Further, Fourier transform infrared (FTIR) and circular dichroism (CD) spectra suggested that RA induces conformational and structural changes in hTf.
Additionally, molecular dynamics (MD) studies were carried out to investigate the stability of the hTf and hTf–RA system and suggested that binding of RA induces structural alteration in hTf with free hTf being more stable.
This study provides a rationale to use RA in drug development against neurodegenerative disorders by designing novel functional foods containing RA.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shamsi, Anas& Anwar, Saleha& Shahbaaz, Mohd& Mohammad, Taj& Al-Ajmi, Mohamed F.& Hussain, Afzal…[et al.]. 2020. Evaluation of Binding of Rosmarinic Acid with Human Transferrin and Its Impact on the Protein Structure: Targeting Polyphenolic Acid-Induced Protection of Neurodegenerative Disorders. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1203646
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shamsi, Anas…[et al.]. Evaluation of Binding of Rosmarinic Acid with Human Transferrin and Its Impact on the Protein Structure: Targeting Polyphenolic Acid-Induced Protection of Neurodegenerative Disorders. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1203646
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shamsi, Anas& Anwar, Saleha& Shahbaaz, Mohd& Mohammad, Taj& Al-Ajmi, Mohamed F.& Hussain, Afzal…[et al.]. Evaluation of Binding of Rosmarinic Acid with Human Transferrin and Its Impact on the Protein Structure: Targeting Polyphenolic Acid-Induced Protection of Neurodegenerative Disorders. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1203646
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1203646
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر