Klotho Alleviates Lung Injury Caused by Paraquat via Suppressing ROSP38 MAPK-Regulated Inflammatory Responses and Apoptosis
المؤلفون المشاركون
Zhang, Zhiqiang
Nian, Qing
Chen, Gang
Cui, Shuqing
Han, Yuzhen
Zhang, Jinying
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-05-14
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Acute lung injury (ALI) induced by paraquat (PQ) progresses rapidly with high mortality; however, there is no effective treatment, and the specific mechanism is not well understood.
The antiaging protein klotho (KL) has multiple functions and exerts significant influences on various pathophysiological processes.
This work evaluated the impact of KL on PQ-induced ALI and investigated its underlying mechanisms.
As for in vivo research, C57BL/6 mice were treated with PQ (30 mg/kg) intraperitoneal (IP) injection to create a toxicity model of ALI (PQ group).
The mice were divided into control group, KL group, PQ group, and PQ+KL group.
For in vitro experiment, A549 cells were incubated with or without KL and then treated in the presence or absence of PQ for 24 h.
In vivo result indicated that KL reduced the mortality, reduced IL-1β and IL-6 in the bronchoalveolar lavage fluid (BALF), attenuated ALI, and decreased apoptosis in situ.
In vitro result revealed that KL significantly improved cell viability, reduced the levels of IL-1β and IL-6 in culture supernatants, suppressed cell apoptosis, inhibited caspase-3 activation, and enhanced mitochondrial membrane potential (ΔΨm) after PQ treatment.
Besides, KL effectively abated reactive oxygen species (ROS) production, improved GSH content, and lowered lipid peroxidation in PQ-exposed A549 cells.
Further experiments indicated that phosphorylated JNK and P38 MAPK was increased after PQ treatment; however, KL pretreatment could significantly lower the phosphorylation of P38 MAPK.
Suppression of P38 MAPK improved cell viability, alleviated inflammatory response, and reduced apoptosis-related signals; however, it had no obvious effect on the production of ROS.
Treatment with N-acetylcysteine (NAC), a classic ROS scavenger, could suppress ROS production and P38 MAPK activation.
These findings suggested that KL could alleviate PQ-caused ALI via inhibiting ROS/P38 MAPK signaling-regulated inflammatory responses and mitochondria-dependent apoptosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Zhiqiang& Nian, Qing& Chen, Gang& Cui, Shuqing& Han, Yuzhen& Zhang, Jinying. 2020. Klotho Alleviates Lung Injury Caused by Paraquat via Suppressing ROSP38 MAPK-Regulated Inflammatory Responses and Apoptosis. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1203840
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Zhiqiang…[et al.]. Klotho Alleviates Lung Injury Caused by Paraquat via Suppressing ROSP38 MAPK-Regulated Inflammatory Responses and Apoptosis. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1203840
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Zhiqiang& Nian, Qing& Chen, Gang& Cui, Shuqing& Han, Yuzhen& Zhang, Jinying. Klotho Alleviates Lung Injury Caused by Paraquat via Suppressing ROSP38 MAPK-Regulated Inflammatory Responses and Apoptosis. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1203840
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1203840
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر