Identifying Therapeutic Targets for Sepsis Research: A Characterization Study of the Inflammatory Players in the Cecal Ligation and Puncture Model
المؤلفون المشاركون
Ebo, Didier
De Winter, Benedicte
Bridts, Chris
Francque, Sven
Nullens, Sara
De Man, Joris
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-08-05
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
During sepsis, disturbed gastrointestinal motility and increased mucosal permeability can aggravate sepsis due to the increased risk of bacterial translocation.
To help identify new therapeutic targets, there is a need for animal models that mimic the immunological changes in the gastrointestinal tract as observed during human sepsis.
We therefore characterized in detail the gastrointestinal neuroimmune environment in the cecal ligation and puncture (CLP) model, which is the gold standard animal model of microbial sepsis.
Mice were sacrificed at day 2 and day 7, during which gastrointestinal motility was assessed and cytokines were measured in the serum and the colon.
In the spleen, lymph nodes, ileum, and colon, subsets of leukocyte populations were identified by flow cytometry.
Septic animals displayed an impaired gastrointestinal motility at day 2 and day 7.
Two days post-CLP, increased serum and colonic levels of proinflammatory cytokines were measured.
Flow cytometry revealed an influx of neutrophils in the colon and ileum, increased numbers of macrophages in the spleen and mesenteric lymph nodes, and an enhanced number of mast cells in all tissues.
At day 7 post-CLP, lymphocyte depletion was observed in all tissues coinciding with increased IL-10 and TGF-β levels, as well as increased colonic levels of IL-17A and IFN-γ.
Thus, CLP-induced sepsis in mice results in simultaneous activation of pro- and anti-inflammatory players at day 2 and day 7 in different tissues, mimicking human sepsis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nullens, Sara& De Man, Joris& Bridts, Chris& Ebo, Didier& Francque, Sven& De Winter, Benedicte. 2018. Identifying Therapeutic Targets for Sepsis Research: A Characterization Study of the Inflammatory Players in the Cecal Ligation and Puncture Model. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1203856
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nullens, Sara…[et al.]. Identifying Therapeutic Targets for Sepsis Research: A Characterization Study of the Inflammatory Players in the Cecal Ligation and Puncture Model. Mediators of Inflammation No. 2018 (2018), pp.1-17.
https://search.emarefa.net/detail/BIM-1203856
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nullens, Sara& De Man, Joris& Bridts, Chris& Ebo, Didier& Francque, Sven& De Winter, Benedicte. Identifying Therapeutic Targets for Sepsis Research: A Characterization Study of the Inflammatory Players in the Cecal Ligation and Puncture Model. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1203856
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1203856
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر