The Effect and Mechanism of TLR9KLF4 in FFA-Induced Adipocyte Inflammation
المؤلفون المشاركون
Han, Bo
Zhang, Jun
Xie, Jianxin
Wang, Cuizhe
Ha, Xiaodan
Li, Wei
Gu, Yajuan
Zhang, Meixiu
Wu, Jinxiu
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-12-18
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Objective.
Current research has reported that obesity is a chronic inflammatory state, which is closely related with excessive accumulation of free fatty acid, while the specific mechanism that high level of FFA causes inflammation is not very clear.
Thus, our research intended to observe the high FFA effects on TLR9/KLF4 expression and the downstream inflammatory factors, to explore the mechanism of inflammatory response suppressed by TLR9/KLF4.
Methods.
qRT-PCR and Western blot were used to detect the mRNA and protein expression levels of TLR9, KLF4, and key inflammation-related factors.
ELISA was used to detect the release level of inflammatory cytokines.
The triglyceride (TG) and glucose (GLU) testing cassettes were used to detect the TG and GLU levels in culture medium.
Results.
In the omental tissue of obese individuals (OB), we found that TLR9, KLF4, mRNA, and the protein expression levels were lower than those of the normal weight control (NC) group.
Similarly, in the omental tissue of high-fat diet (HFD) rats, we found that the mRNA expression levels of TLR9 and KLF4 were lower than those of the normal diet control group.
In mature adipocytes, we found that KLF4 played an important anti-inflammatory role; moreover, PA can promote the development of inflammation by inhibiting KLF4 expression; TLR9 has a positive regulation function on KLF4 expression, but unrelated to PA.
Conclusions.
TLR9/KLF4 is involved in regulating FFA-induced adipocyte inflammation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Cuizhe& Zhang, Meixiu& Wu, Jinxiu& Li, Wei& Ha, Xiaodan& Gu, Yajuan…[et al.]. 2018. The Effect and Mechanism of TLR9KLF4 in FFA-Induced Adipocyte Inflammation. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1204014
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Cuizhe…[et al.]. The Effect and Mechanism of TLR9KLF4 in FFA-Induced Adipocyte Inflammation. Mediators of Inflammation No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1204014
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Cuizhe& Zhang, Meixiu& Wu, Jinxiu& Li, Wei& Ha, Xiaodan& Gu, Yajuan…[et al.]. The Effect and Mechanism of TLR9KLF4 in FFA-Induced Adipocyte Inflammation. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1204014
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1204014
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر