Lactobacillus reuteri LR1 Improved Expression of Genes of Tight Junction Proteins via the MLCK Pathway in IPEC-1 Cells during Infection with Enterotoxigenic Escherichia coli K88
المؤلفون المشاركون
Wang, Zhilin
Ma, Xianyong
Yi, Hongbo
Wang, Li
Xiong, Yunxia
Qiu, Yueqin
Wen, Xiaolu
Jiang, Zongyong
Yang, Xuefen
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-08-19
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Intestinal epithelial barrier damage disrupts immune homeostasis and leads to many intestinal disorders.
Lactobacillus reuteri strains have probiotic functions in their modulation of the microbiota and immune system in intestines.
In this study, the effects of L.
reuteri LR1, a new strain isolated from the feces of weaning piglets, on intestinal epithelial barrier damage in IPEC-1 cells caused by challenge with enterotoxigenic Escherichia coli (ETEC) K88 were examined.
It was found that L.
reuteri LR1, in large part, offset the ETEC K88-induced increase in permeability of IPEC-1 cell monolayers and decreased the adhesion and invasion of the coliform in IPEC-1 cells.
In addition, L.
reuteri LR1 increased transcript abundance and protein contents of tight junction (TJ) proteins zonula occluden-1 (ZO-1) and occludin in ETEC K88-infected IPEC-1 cells, whereas it had no effects on claudin-1 and F-actin expression.
Using colloidal gold immunoelectron microscopy, these effects of L.
reuteri LR1 on ZO-1 and occludin content in IPEC-1 cells were confirmed.
By using ML-7, a selective inhibitor of myosin light-chain kinase (MLCK), the beneficial effect of L.
reuteri LR1 on contents of ZO-1 and occludin was shown to be dependent on the MLCK pathway.
In conclusion, L.
reuteri LR1 had beneficial effects on epithelial barrier function consistent with increasing ZO-1 and occludin expression via a MLCK-dependent manner in IPEC-1 cells during challenge with ETEC K88.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yi, Hongbo& Wang, Li& Xiong, Yunxia& Wang, Zhilin& Qiu, Yueqin& Wen, Xiaolu…[et al.]. 2018. Lactobacillus reuteri LR1 Improved Expression of Genes of Tight Junction Proteins via the MLCK Pathway in IPEC-1 Cells during Infection with Enterotoxigenic Escherichia coli K88. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1204024
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yi, Hongbo…[et al.]. Lactobacillus reuteri LR1 Improved Expression of Genes of Tight Junction Proteins via the MLCK Pathway in IPEC-1 Cells during Infection with Enterotoxigenic Escherichia coli K88. Mediators of Inflammation No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1204024
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yi, Hongbo& Wang, Li& Xiong, Yunxia& Wang, Zhilin& Qiu, Yueqin& Wen, Xiaolu…[et al.]. Lactobacillus reuteri LR1 Improved Expression of Genes of Tight Junction Proteins via the MLCK Pathway in IPEC-1 Cells during Infection with Enterotoxigenic Escherichia coli K88. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1204024
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1204024
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر