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Nephroprotective Effect of Embryonic Stem Cells Reducing Lipid Peroxidation in Kidney Injury Induced by Cisplatin
المؤلفون المشاركون
Rojas-López, Marlon
Vazquez-Zapien, Gustavo Jesus
Mata-Miranda, Monica Maribel
Bernal-Barquero, Carlos Eduardo
Martinez-Cuazitl, Adriana
Guerrero-Robles, Carla Ivonne
Sánchez Monroy, Virginia
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-03-14
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Introduction.
The acute kidney injury (AKI) is characterized by a sudden glomerular filtration reduction.
Renal or intrinsic causes of AKI include nephrotoxicity induced by exogenous agents like cisplatin, which causes oxidative stress altering the biochemical process and leading to apoptosis.
Therefore, this research is aimed at analyzing the embryonic stem cells (ESC) nephroprotective effect in AKI induced by cisplatin, employing genetic, phenotypic, and microspectroscopic techniques.
Methods.
Thirty mice were randomly divided into three groups (n=10): the healthy, isotonic salt solution (ISS), and mouse embryonic stem cells (mESC) groups.
The ISS and mESC groups were subjected to AKI using cisplatin; 24 h post-AKI received an intraperitoneal injection of ISS or 1×106 mESC, respectively.
At days 4 and 8 post-AKI, five mice of each group were sacrificed to analyze the histopathological, genetic (PDK4 and HO-1), protein (p53), and vibrational microspectroscopic changes.
Results.
Histopathologically, interstitial nephritis and acute tubular necrosis were observed; however, the mESC group showed a more preserved microarchitecture with high cellularity.
Additionally, the PDK4 and HO-1 gene expression only increased in the ISS group on day 4 post-AKI.
Likewise, p53 was more immunoexpressed at day 8 post-AKI in the ISS group.
About biomolecular analysis by microspectroscopy, bands associated with lipids, proteins, and nucleic acids were evidenced.
Besides, ratios related to membrane function (protein/lipid), unsaturated lipid content (olefinic/total lipid, olefinic/total CH2, and CH2/CH3), and lipid peroxidation demonstrated oxidative stress induction and lipid peroxidation increase mainly in the ISS group.
Finally, the principal component analysis discriminated against each group; nonetheless, some data of the healthy and mESC groups at day 8 were correlated.
Conclusions.
The mESC implant diminishes cisplatin nephrotoxicity, once the protective effect in the reduction of lipid peroxidation was demonstrated, reflecting a functional and histological restoration.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mata-Miranda, Monica Maribel& Bernal-Barquero, Carlos Eduardo& Martinez-Cuazitl, Adriana& Guerrero-Robles, Carla Ivonne& Sánchez Monroy, Virginia& Rojas-López, Marlon…[et al.]. 2019. Nephroprotective Effect of Embryonic Stem Cells Reducing Lipid Peroxidation in Kidney Injury Induced by Cisplatin. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1204149
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mata-Miranda, Monica Maribel…[et al.]. Nephroprotective Effect of Embryonic Stem Cells Reducing Lipid Peroxidation in Kidney Injury Induced by Cisplatin. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1204149
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mata-Miranda, Monica Maribel& Bernal-Barquero, Carlos Eduardo& Martinez-Cuazitl, Adriana& Guerrero-Robles, Carla Ivonne& Sánchez Monroy, Virginia& Rojas-López, Marlon…[et al.]. Nephroprotective Effect of Embryonic Stem Cells Reducing Lipid Peroxidation in Kidney Injury Induced by Cisplatin. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1204149
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1204149
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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