Suppression of LPS-Induced Inflammation by Chalcone Flavokawain A through Activation of Nrf2ARE-Mediated Antioxidant Genes and Inhibition of ROSNFκB Signaling Pathways in Primary Splenocytes
المؤلفون المشاركون
Hseu, You Cheng
Liao, Jiunn-Wang
Yang, Hsin-Ling
Huang, Pei-Jane
Gowrisankar, Yugandhar Vudhya
Yang, Ting-Yu
Liao, Chun-Huei
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-13
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Oxidative stress is an important contributing factor for inflammation.
Piper methysticum, also known as Kava-kava, is a shrub whose root extract has been consumed as a drink by the pacific islanders for a long time.
Flavokawain A (FKA) is a novel chalcone derived from the kava plant that is known to have medicinal properties.
This study was aimed at demonstrating the antioxidant molecular mechanisms mediated by FKA on lipopolysaccharide- (LPS-) induced inflammation in BALB/c mouse-derived primary splenocytes.
In vitro data show that the nontoxic concentrations of FKA (2-30 μM) significantly suppressed the proinflammatory cytokine (TNF-α, IL-1β, and IL-6) release but induced the secretion of interleukin-10 (IL-10), an anti-inflammatory cytokine.
It was also shown that FKA pretreatment significantly downregulated the LPS-induced ROS production and blocked the activation of the NFκB (p65) pathway leading to the significant suppression of iNOS, COX-2, TNF-α, and IL-1β protein expressions.
Notably, FKA favored the nuclear translocation of Nrf2 leading to the downstream expression of antioxidant proteins HO-1, NQO-1, and γ-GCLC via the Nrf2/ARE signaling pathway signifying the FKA’s potent antioxidant mechanism in these cells.
Supporting the in vitro data, the ex vivo data obtained from primary splenocytes derived from the FKA-preadministered BALB/c mice (orally) show that FKA significantly suppressed the proinflammatory cytokine (TNF-α, IL-1β, and IL-6) secretion in control-, LPS-, or Concanavalin A- (Con A-) stimulated cells.
A significant decrease in the ratios of pro- and anti-inflammatory cytokines (IL-6/IL-10; TNF-α/IL-10) showed that FKA possesses strong anti-inflammatory properties.
Furthermore, BALB/c mice induced with experimental pancreatitis using cholecystokinin- (CCK-) 8 showed decreased serum lipase levels due to FKA pretreatment.
We conclude that with its potent antioxidant and anti-inflammatory properties, chalcone flavokawain A could be a novel therapeutic agent in the treatment of inflammation-associated diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Hsin-Ling& Yang, Ting-Yu& Gowrisankar, Yugandhar Vudhya& Liao, Chun-Huei& Liao, Jiunn-Wang& Huang, Pei-Jane…[et al.]. 2020. Suppression of LPS-Induced Inflammation by Chalcone Flavokawain A through Activation of Nrf2ARE-Mediated Antioxidant Genes and Inhibition of ROSNFκB Signaling Pathways in Primary Splenocytes. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1204264
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Hsin-Ling…[et al.]. Suppression of LPS-Induced Inflammation by Chalcone Flavokawain A through Activation of Nrf2ARE-Mediated Antioxidant Genes and Inhibition of ROSNFκB Signaling Pathways in Primary Splenocytes. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1204264
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Hsin-Ling& Yang, Ting-Yu& Gowrisankar, Yugandhar Vudhya& Liao, Chun-Huei& Liao, Jiunn-Wang& Huang, Pei-Jane…[et al.]. Suppression of LPS-Induced Inflammation by Chalcone Flavokawain A through Activation of Nrf2ARE-Mediated Antioxidant Genes and Inhibition of ROSNFκB Signaling Pathways in Primary Splenocytes. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1204264
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1204264
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر