IKK Epsilon Deficiency Attenuates Angiotensin II-Induced Abdominal Aortic Aneurysm Formation in Mice by Inhibiting Inflammation, Oxidative Stress, and Apoptosis
المؤلفون المشاركون
Chai, Hao
Tao, ZhongHao
Qi, YongChao
Qi, HaoYu
Chen, Wen
Xu, YueYue
Zhang, LeiYang
Chen, HongWei
Chen, Xin
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-01-23
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
Abdominal aortic aneurysm (AAA) is a vascular disorder that is considered a chronic inflammatory disease.
However, the precise molecular mechanisms involved in AAA have not been fully elucidated.
Recently, significant progress has been made in understanding the function and mechanism of action of inhibitor of kappa B kinase epsilon (IKKε) in inflammatory and metabolic diseases.
The angiotensin II- (Ang II-) induced or pharmacological inhibitors were established to test the effects of IKKε on AAA in vivo.
After mice were continuously stimulated with Ang II for 28 days, morphologically, we found that knockout of IKKε reduced AAA formation and drastically reduced maximal diameter and severity.
We also observed a decrease in elastin degradation and medial destruction, which were independent of systolic blood pressure or plasma cholesterol concentrations.
Western blot analyses and immunohistochemical staining were carried out to measure IKKε expression in AAA tissues and cell lines.
AAA phenotype of mice was measured by ultrasound and biochemical indexes.
In zymography, immunohistology staining, immunofluorescence staining, and reactive oxygen species (ROS) analysis, TUNEL assay was used to examine the effects of IKKε on AAA progression in AAA mice.
IKKε deficiency significantly inhibited inflammatory macrophage infiltration, matrix metalloproteinase (MMP) activity, ROS production, and vascular smooth muscle cell (VSMC) apoptosis.
We used primary mouse aortic VSMC isolated from apolipoprotein E (Apoe) −/− and Apoe−/−IKKε−/− mice.
Mechanistically, IKKε deficiency blunted the activation of the ERK1/2 pathway.
The IKKε inhibitor, amlexanox, has the same impact in AAA.
Our results demonstrate a critical role of IKKε in AAA formation induced by Ang II in Apoe−/− mice.
Targeting IKKε may constitute a novel therapeutic strategy to prevent AAA progression.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chai, Hao& Tao, ZhongHao& Qi, YongChao& Qi, HaoYu& Chen, Wen& Xu, YueYue…[et al.]. 2020. IKK Epsilon Deficiency Attenuates Angiotensin II-Induced Abdominal Aortic Aneurysm Formation in Mice by Inhibiting Inflammation, Oxidative Stress, and Apoptosis. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1204310
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chai, Hao…[et al.]. IKK Epsilon Deficiency Attenuates Angiotensin II-Induced Abdominal Aortic Aneurysm Formation in Mice by Inhibiting Inflammation, Oxidative Stress, and Apoptosis. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-16.
https://search.emarefa.net/detail/BIM-1204310
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chai, Hao& Tao, ZhongHao& Qi, YongChao& Qi, HaoYu& Chen, Wen& Xu, YueYue…[et al.]. IKK Epsilon Deficiency Attenuates Angiotensin II-Induced Abdominal Aortic Aneurysm Formation in Mice by Inhibiting Inflammation, Oxidative Stress, and Apoptosis. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1204310
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1204310
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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