Internalization and Transportation of Endothelial Cell Surface KCa2.3 and KCa3.1 in Normal Pregnancy and Preeclampsia
المؤلفون المشاركون
Oh, Seikwan
Choi, Shinkyu
Kim, Ji Aee
Park, Mi Hye
Cho, Geum Joon
Suh, Suk Hyo
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-23
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Altered redox state modulates the expression levels of endothelial KCa2.3 and KCa3.1 (KCas) in normal pregnancy (NP) and preeclampsia (PE), thereby regulating vascular contractility.
The mechanisms underlying KCas endocytosis and transportation remain unknown.
We investigated the regulation of KCas expression in plasma membrane (PM) during NP and PE.
Cultured human uterine artery endothelial cells were incubated in serum from normal nonpregnant women and women with NP or PE, or in oxidized LDL-, or lysophosphatidylcholine- (LPC-) containing a medium for 24 hours.
NP serum elevated PM levels of KCas and reduced caveolin-1 and clathrin levels.
PE serum, oxidized LDL, or LPC reduced PM levels of KCas and elevated caveolin-1, clathrin, Rab5c, and early endosome antigen-1 (EEA1) levels.
Reduced KCas levels by PE serum or LPC were reversed by inhibition of caveolin-1, clathrin, or EEA1.
Catalase and glutathione peroxidase 1 (GPX1) knockdown elevated PM-localized KCas levels and reduced caveolin-1 and clathrin levels.
Elevated KCa2.3 levels upon catalase and GPX1 knockdown were reversed by PEG-catalase treatment.
An H2O2 donor reduced clathrin and Rab5c.
In contrast, elevated clathrin, caveolin-1, or colocalization of caveolin-1 with KCa3.1 by PE serum or LPC was reversed by NADPH oxidase inhibitors or antioxidants.
A superoxide donor xanthine+xanthine oxidase elevated caveolin-1 or Rab5c levels.
We concluded that KCas are endocytosed in a caveola- or a clathrin-dependent manner and transported in a Rab5c- and EEA1-dependent manner during pregnancy.
The endocytosis and transportation processes may slow down via H2O2-mediated pathways in NP and may be accelerated via superoxide-mediated pathways in PE.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Choi, Shinkyu& Kim, Ji Aee& Oh, Seikwan& Park, Mi Hye& Cho, Geum Joon& Suh, Suk Hyo. 2019. Internalization and Transportation of Endothelial Cell Surface KCa2.3 and KCa3.1 in Normal Pregnancy and Preeclampsia. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1204319
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Choi, Shinkyu…[et al.]. Internalization and Transportation of Endothelial Cell Surface KCa2.3 and KCa3.1 in Normal Pregnancy and Preeclampsia. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1204319
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Choi, Shinkyu& Kim, Ji Aee& Oh, Seikwan& Park, Mi Hye& Cho, Geum Joon& Suh, Suk Hyo. Internalization and Transportation of Endothelial Cell Surface KCa2.3 and KCa3.1 in Normal Pregnancy and Preeclampsia. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1204319
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1204319
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر