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Intrauterine Malnutrition Reduced Long Leptin Receptor Isoform Expression and Proinflammatory Cytokine Production in Male Rat Pulmonary Endothelial Cells Stimulated by Lipopolysaccharide
المؤلفون المشاركون
Landgraf, Richardt G.
Balbino, Aleksandro Martins
Silva, Marina M.
Azevedo, Gabriela A.
Gil, Noemi L.
Ferreira, Renaide R.
dos Santos, Leila A.
Gasparin, Rebéca M.
Fernandes, Liliam
Landgraf, Maristella A.
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-07-09
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Background/Aims.
We have previously shown that low birth weight (LBW) rats exposed to intrauterine malnutrition have an impaired lung inflammatory response and reduced levels of inflammatory mediators; however, circulating leptin levels were not increased.
We evaluated long leptin receptor isoform (ObRb) expression in lung endothelial cells from low birth weight rats and examined its role in the production of lipid mediators and cytokines.
Methods.
Lung endothelial cells were obtained from normal birth weight (NBW) rats or LBW rats subjected to intrauterine malnutrition.
These cells were stimulated with leptin (10 ng/mL), LPS (lipopolysaccharide, 1 μg/mL), or leptin plus LPS.
Six hours after stimulation, the production of inflammatory mediators (PGE2, LTB4, IL-1β, and IL-6) was evaluated using commercial ELISA kits, and Western blotting was performed to investigate p38MAPK, NF-κB, and ObRb expression.
Results.
Leptin increased IL-1β levels in only cells from the NBW group, whereas LPS increased PGE2 and LTB4 levels in cells from both groups; leptin addition potentiated lipid mediator production induced by LPS in the NBW group.
LPS enhanced the production of IL-1β and IL-6 in only endothelial cells from NBW rats.
Leptin receptor expression was decreased (63%) in endothelial cells from LBW rats.
None of the stimuli increased NF-κB or p38 signaling pathway expression in cells from LBW rats.
Conclusion.
These results suggest that intrauterine malnutrition compromises leptin receptor expression and cytokine production in pulmonary endothelial cells stimulated by LPS; these effects seem to involve the NF-κB and p38MAPK signaling pathways.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Balbino, Aleksandro Martins& Silva, Marina M.& Azevedo, Gabriela A.& Gil, Noemi L.& Ferreira, Renaide R.& dos Santos, Leila A.…[et al.]. 2018. Intrauterine Malnutrition Reduced Long Leptin Receptor Isoform Expression and Proinflammatory Cytokine Production in Male Rat Pulmonary Endothelial Cells Stimulated by Lipopolysaccharide. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1204345
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Balbino, Aleksandro Martins…[et al.]. Intrauterine Malnutrition Reduced Long Leptin Receptor Isoform Expression and Proinflammatory Cytokine Production in Male Rat Pulmonary Endothelial Cells Stimulated by Lipopolysaccharide. Mediators of Inflammation No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1204345
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Balbino, Aleksandro Martins& Silva, Marina M.& Azevedo, Gabriela A.& Gil, Noemi L.& Ferreira, Renaide R.& dos Santos, Leila A.…[et al.]. Intrauterine Malnutrition Reduced Long Leptin Receptor Isoform Expression and Proinflammatory Cytokine Production in Male Rat Pulmonary Endothelial Cells Stimulated by Lipopolysaccharide. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1204345
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1204345
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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