![](/images/graphics-bg.png)
Shape-Depended Biological Properties of Ag3PO4 Microparticles: Evaluation of Antimicrobial Properties and Cytotoxicity in In Vitro Model—Safety Assessment of Potential Clinical Usage
المؤلفون المشاركون
Zaleska-Medynska, Adriana
Steckiewicz, Karol P.
Zwara, Julia
Jaskiewicz, Maciej
Kowalski, Szymon
Inkielewicz-Stepniak, Iwona
Kamysz, Wojciech
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-19، 19ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-20
دولة النشر
مصر
عدد الصفحات
19
التخصصات الرئيسية
الملخص EN
Implant-related infections are an emerging clinical and economic problem.
Therefore, we decided to assess potential clinical usefulness and safety of silver orthophosphate microparticles (SOMPs) regarding their shape.
We synthesized and then assessed antimicrobial properties and potential cytotoxicity of six shapes of SOMPs (tetrapod, cubes, spheres, tetrahedrons, branched, and rhombic dodecahedron).
We found that SOMPs had a high antimicrobial effect; they were more efficient against fungi than bacteria.
SOMPs exerted an antimicrobial effect in concentrations not toxic to mammalian cells: human fetal osteoblast (hFOB1.19), osteosarcoma (Saos-2), mouse preosteoblasts (MC3T3-E1), skin fibroblast (HDF), and mouse myoblast (C2C12).
At higher concentration SOMPs, induced shape- and concentration-dependent cytotoxicity (according to MTT and BrdU assays).
Tetrapod SOMPs had the smallest effect, whereas cubical SOMPs, the highest on cell viability.
hFOB1.19 were the most resistant cells and C2C12, the most susceptible ones.
We have proven that the induction of oxidative stress and inflammation is involved in the cytotoxic mechanism of SOMPs.
After treatment with microparticles, we observed changes in levels of reactive oxygen species, first-line defense antioxidants-superoxide dismutase (SOD1, SOD3), and glutathione peroxidase (GPX4), metalloproteinase (MMP1, MMP3), and NF-κB protein.
Neither cell cycle distribution nor ultrastructure was altered as determined by flow cytometry and transmission electron microscopy, respectively.
In conclusion, silver orthophosphate may be a safe and effective antimicrobial agent on the implant surface.
Spherical-shaped SOMPs are the most promising for biomedical application.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Steckiewicz, Karol P.& Zwara, Julia& Jaskiewicz, Maciej& Kowalski, Szymon& Kamysz, Wojciech& Zaleska-Medynska, Adriana…[et al.]. 2019. Shape-Depended Biological Properties of Ag3PO4 Microparticles: Evaluation of Antimicrobial Properties and Cytotoxicity in In Vitro Model—Safety Assessment of Potential Clinical Usage. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-19.
https://search.emarefa.net/detail/BIM-1204777
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Steckiewicz, Karol P.…[et al.]. Shape-Depended Biological Properties of Ag3PO4 Microparticles: Evaluation of Antimicrobial Properties and Cytotoxicity in In Vitro Model—Safety Assessment of Potential Clinical Usage. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-19.
https://search.emarefa.net/detail/BIM-1204777
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Steckiewicz, Karol P.& Zwara, Julia& Jaskiewicz, Maciej& Kowalski, Szymon& Kamysz, Wojciech& Zaleska-Medynska, Adriana…[et al.]. Shape-Depended Biological Properties of Ag3PO4 Microparticles: Evaluation of Antimicrobial Properties and Cytotoxicity in In Vitro Model—Safety Assessment of Potential Clinical Usage. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-19.
https://search.emarefa.net/detail/BIM-1204777
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1204777
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)