Nrf2 Overexpression for the Protective Effect of Skin-Derived Precursors against UV-Induced Damage: Evidence from a Three-Dimensional Skin Model

الملخص EN

Background.

Skin photodamage is associated with ultraviolet- (UV-) induced reactive oxygen species (ROS) overproduction and nuclear factor erythroid 2-related factor 2 (Nrf2) inactivation.

In our previous study, skin-derived precursors (SKPs) were shown to ameliorate a UV-induced damage in mice, probably through Nrf2 activation and ROS scavenging.

Objective.

To clarify the mechanism underlying the photoprotective effect of SKPs against UV-induced damage in a three-dimensional (3D) skin model.

Methods.

The Nrf2 gene in SKPs was modified using lentiviral infection, and 3D skin models were reconstructed with keratinocytes and fibroblasts on the basis of type I collagen.

Subsequently, these models were divided into the following six groups: normal, model, overexpressed, control, silenced, and negative control groups.

Prior to irradiation, respective SKPs were injected into the last four groups.

Next, all groups except the normal group were exposed to UVA+UVB.

Lastly, the pathological and molecular-biological techniques were employed to determine the parameters.

Additionally, LY294002, a PI3K inhibitor, was used to investigate the roles of PI3K/Akt and Nrf2/hemeoxygenase-1 (HO-1) in SKP photoprotection.

Results.

Normal 3D skin models appeared as milky-white analogs with a clear, well-arranged histological structure.

After the skin was exposed to irradiation, it exhibited cell swelling and a disorganized structure and developed nuclear condensation with numerous apoptotic cells.

The expressions of cellular protective genes and Nrf2/HO-1/PI3K/Akt proteins remarkably decreased, which were accompanied by increased oxidative stress and decreased antioxidants (P<0.05).

However, these phenomena were reversed by nrf2-overexpressing SKPs.

The 3D skin in the overexpressed group showed mild swelling, neatly arranged cells, and few apoptotic cells.

Cellular protective genes and Nrf2/HO-1/PI3K/Akt proteins were highly expressed, and the oxidative biomarkers were remarkably ameliorated (P<0.05).

Nevertheless, the expression of these proteins decreased after LY294002 pretreatment regardless of SKP treatment or not.

Meanwhile, there were increases in both UV-induced apoptotic cells and ROS level accompanied with SOD and GPX decrease in the presence of LY294002.

Conclusions.

Evidence from the 3D skin model demonstrates that the protection of SKPs against UV-mediated damage is primarily via the PI3K/Akt-mediated activation of the Nrf2/HO-1 pathway, indicating that SKPs may be a promising candidate for the treatment of photodermatoses.