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Network Pharmacology Analysis and Molecular Characterization of the Herbal Medicine Formulation Qi-Fu-Yin for the Inhibition of the Neuroinflammatory Biomarker iNOS in Microglial BV-2 Cells: Implication for the Treatment of Alzheimer’s Disease
المؤلفون المشاركون
Wang, Weiwei
Ngo, Fung Yin
Chen, Qilei
Gao, Jin-Ming
Rong, Jianhui
Zhao, Jia
Chen, Hubiao
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-09-01
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Aberrant microglial activation drives neuroinflammation and neurodegeneration in Alzheimer’s disease (AD).
The present study is aimed at investigating whether the herbal formula Qi-Fu-Yin (QFY) could inhibit the inflammatory activation of cultured BV-2 microglia.
A network pharmacology approach was employed to predict the active compounds of QFY, protein targets, and affected pathways.
The representative pathways and molecular functions of the targets were analyzed by Gene Ontology (GO) and pathway enrichment.
A total of 145 active compounds were selected from seven herbal ingredients of QFY.
Targets (e.g., MAPT, APP, ACHE, iNOS, and COX-2) were predicted for the selected active compounds based on the relevance to AD and inflammation.
As a validation, fractions were sequentially prepared by aqueous extraction, ethanolic precipitation, and HPLC separation, and assayed for downregulating two key proinflammatory biomarkers iNOS and COX-2 in lipopolysaccharide- (LPS-) challenged BV-2 cells by the Western blotting technique.
Moreover, the compounds of QFY in 90% ethanol downregulated iNOS in BV-2 cells but showed no activity against COX-2 induction.
Among the herbal ingredients of QFY, Angelicae Sinensis Radix and Ginseng Radix et Rhizoma contributed to the selective inhibition of iNOS induction.
Furthermore, chemical analysis identified ginsenosides, especially Rg3, as antineuroinflammatory compounds.
The herbal formula QFY may ameliorate neuroinflammation via downregulating iNOS in microglia.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ngo, Fung Yin& Wang, Weiwei& Chen, Qilei& Zhao, Jia& Chen, Hubiao& Gao, Jin-Ming…[et al.]. 2020. Network Pharmacology Analysis and Molecular Characterization of the Herbal Medicine Formulation Qi-Fu-Yin for the Inhibition of the Neuroinflammatory Biomarker iNOS in Microglial BV-2 Cells: Implication for the Treatment of Alzheimer’s Disease. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1204946
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ngo, Fung Yin…[et al.]. Network Pharmacology Analysis and Molecular Characterization of the Herbal Medicine Formulation Qi-Fu-Yin for the Inhibition of the Neuroinflammatory Biomarker iNOS in Microglial BV-2 Cells: Implication for the Treatment of Alzheimer’s Disease. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1204946
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ngo, Fung Yin& Wang, Weiwei& Chen, Qilei& Zhao, Jia& Chen, Hubiao& Gao, Jin-Ming…[et al.]. Network Pharmacology Analysis and Molecular Characterization of the Herbal Medicine Formulation Qi-Fu-Yin for the Inhibition of the Neuroinflammatory Biomarker iNOS in Microglial BV-2 Cells: Implication for the Treatment of Alzheimer’s Disease. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1204946
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1204946
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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