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miR-155-5p Promotes Oxalate- and Calcium-Induced Kidney Oxidative Stress Injury by Suppressing MGP Expression
المؤلفون المشاركون
Jiang, Kehua
Hu, Jianxin
Luo, Guangheng
Song, Dalong
Zhang, Peng
Zhu, Jianguo
Sun, Fa
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-03-07
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Oxalate and calcium are the major risk factors for calcium oxalate (CaOx) stone formation.
However, the exact mechanism remains unclear.
This study was designed to confirm the potential function of miR-155-5p in the formation of CaOx induced by oxalate and calcium oxalate monohydrate (COM).
The HK-2 cells were treated by the different concentrations of oxalate and COM for 48 h.
We found that oxalate and COM treatment significantly increased ROS generation, LDH release, cellular MDA levels, and H2O2 concentration in HK-2 cells.
The results of qRT-PCR and western blot showed that expression of NOX2 was upregulated, while that of SOD-2 was downregulated following the treatment with oxalate and COM in HK-2 cells.
Moreover, the results of miRNA microarray analysis showed that miR-155-5p was significantly upregulated after oxalate and COM treated in HK-2 cells, but miR-155-5p inhibitor treatment significantly decreased ROS generation, LDH release, cellular MDA levels, and H2O2 concentration in HK-2 cells incubated with oxalate and COM.
miR-155-5p negatively regulated the expression level of MGP via directly targeting its 3′-UTR, verified by the Dual-Luciferase Reporter System.
In vivo, polarized light optical microphotography showed that CaOx crystal significantly increased in the high-dose oxalate and Ca2+ groups compared to the control group.
Furthermore, IHC analyses showed strong positive staining intensity for the NOX-2 protein in the high-dose oxalate and Ca2+-treated mouse kidneys, and miR-155-5p overexpression can further enhance its expression.
However, the expression of SOD-2 protein was weakly stained.
In conclusion, our study indicates that miR-155-5p promotes oxalate- and COM-induced kidney oxidative stress injury by suppressing MGP expression.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jiang, Kehua& Hu, Jianxin& Luo, Guangheng& Song, Dalong& Zhang, Peng& Zhu, Jianguo…[et al.]. 2020. miR-155-5p Promotes Oxalate- and Calcium-Induced Kidney Oxidative Stress Injury by Suppressing MGP Expression. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1204966
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jiang, Kehua…[et al.]. miR-155-5p Promotes Oxalate- and Calcium-Induced Kidney Oxidative Stress Injury by Suppressing MGP Expression. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1204966
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jiang, Kehua& Hu, Jianxin& Luo, Guangheng& Song, Dalong& Zhang, Peng& Zhu, Jianguo…[et al.]. miR-155-5p Promotes Oxalate- and Calcium-Induced Kidney Oxidative Stress Injury by Suppressing MGP Expression. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1204966
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1204966
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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