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Ethanolic Extract of Dried Leaves from the Cerrado Biome Increases the Cryotolerance of Bovine Embryos Produced In Vitro
المؤلفون المشاركون
Fidelis, Andrei Antonioni Guedes
de Oliveira Fernandes, Gabriela
Melo, Franscislete Rodrigues
Leme, Ligiane de Oliveira
Adona, Paulo Roberto
Kawamoto, Taynan Stonoga
Dode, Margot Alves Nunes
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-23
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
In vitro embryo production (IVP) induces excessive production of reactive oxygen species (ROS), which affects blastocyst quality.
Therefore, the supplementation of culture media with antioxidants is an alternative to overcome oxidative stress damage.
However, there is a growing demand for the use of antioxidant compounds that are more natural and less toxic in cell cultures.
The present study is aimed at evaluating the effect of ethanolic extracts from cerrado leaves on IVP.
First, the antioxidant capacity and the amount of phenolic compounds of the leaves were evaluated.
Then, the best ethanolic extract concentration composed of cagaita (Eugenia dysenterica) and murici (Byrsonima crassifolia) to be used during the in vitro culture of in vitro-produced embryos was determined.
Afterward, we evaluated the influence of the extract of both plants on ROS and glutathione (GSH) production, while also evaluating the apoptosis and ROS metabolism gene expression.
In a subsequent step, the effect of the ethanolic extracts of dried cagaita and murici leaves during embryonic cultivation on the cryotolerance of expanded blastocysts was studied.
The results showed a significant reduction in the proportion of apoptotic cells from embryos cultivated with 0.01 mg/mL of the cagaita ethanolic extract, besides inducing an increase in the GPX4 and PRDX3 transcription levels.
The murici ethanolic extract induced an increase in the transcription abundance of these genes but did not reduce the proportion of apoptotic cells.
In addition, expanded blastocysts cultivated with extracts at a concentration of 0.01 mg/mL and cryopreserved had higher hatching rates and lower degeneration rates when compared to the frozen group previously supplemented with the extracts.
Moreover, the apoptosis rate of embryos cultured for 12 h after cryopreservation was lower in groups previously exposed to extracts during in vitro cultivation.
Such extracts may be used as alternatives to increase the cryotolerance of in vitro-produced embryos.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Fidelis, Andrei Antonioni Guedes& de Oliveira Fernandes, Gabriela& Melo, Franscislete Rodrigues& Leme, Ligiane de Oliveira& Adona, Paulo Roberto& Kawamoto, Taynan Stonoga…[et al.]. 2020. Ethanolic Extract of Dried Leaves from the Cerrado Biome Increases the Cryotolerance of Bovine Embryos Produced In Vitro. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1205019
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Fidelis, Andrei Antonioni Guedes…[et al.]. Ethanolic Extract of Dried Leaves from the Cerrado Biome Increases the Cryotolerance of Bovine Embryos Produced In Vitro. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-16.
https://search.emarefa.net/detail/BIM-1205019
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Fidelis, Andrei Antonioni Guedes& de Oliveira Fernandes, Gabriela& Melo, Franscislete Rodrigues& Leme, Ligiane de Oliveira& Adona, Paulo Roberto& Kawamoto, Taynan Stonoga…[et al.]. Ethanolic Extract of Dried Leaves from the Cerrado Biome Increases the Cryotolerance of Bovine Embryos Produced In Vitro. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1205019
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1205019
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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