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Kaji-Ichigoside F1 and Rosamultin Protect Vascular Endothelial Cells against Hypoxia-Induced Apoptosis via the PI3KAKT or ERK12 Signaling Pathway
المؤلفون المشاركون
Li, Jianyu
Shi, Chaofeng
Zhan, Li
Wu, Yuqiang
Li, Zhengchao
Li, Yaxiao
Wei, Jinxia
Zhang, Yongliang
Li, Lingzhi
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-03-30
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
As a pair of differential isomers, Kaji-ichigoside F1 and Rosamultin are both pentacyclic triterpenoids isolated from the subterranean root of Potentilla anserina L., a plant used in folk medicine in western China as antihypoxia and anti-inflammatory treatments.
We demonstrated that Kaji-ichigoside F1 and Rosamultin effectively prevented hypoxia-induced apoptosis in vascular endothelial cells.
We established a hypoxia model, using EA.hy926 cells, to further explore the mechanisms.
Hypoxia promoted the phosphorylation of AKT, ERK1/2, and NF-κB.
In hypoxic cells treated with Kaji-ichigoside F1, p-ERK1/2 and p-NF-κB levels were increased, while the level of p-AKT was decreased.
Treatment with Rosamultin promoted phosphorylation of ERK1/2, NF-κB, and AKT in hypoxic cells.
Following the addition of LY294002, the levels of p-AKT, p-ERK1/2, and p-NF-κB decreased significantly.
Addition of PD98059 resulted in reduced levels of p-ERK1/2 and p-NF-κB, while p-AKT levels were increased.
Pharmacodynamic analysis demonstrated that both LY294002 and PD98059 significantly inhibited the positive effects of Kaji-ichigoside F1 on cell viability during hypoxia, consistent with the results of hematoxylin-eosin (H&E) staining, DAPI staining, and flow cytometry.
The antihypoxia effects of Rosamultin were remarkably inhibited by LY294002 but promoted by PD98059.
In Kaji-ichigoside F1- and Rosamultin-treated cells, Bcl2 expression was significantly upregulated, while expression of Bax and cytochrome C and levels of cleaved caspase-9 and cleaved caspase-3 were reduced.
Corresponding to pharmacodynamic analysis, LY294002 inhibited the regulatory effects of Kaji-ichigoside F1 and Rosamultin on the above molecules, while PD98059 inhibited the regulatory effects of Kaji-ichigoside F1 but enhanced the regulatory effects of Rosamultin.
In conclusion, Kaji-ichigoside F1 protected vascular endothelial cells against hypoxia-induced apoptosis by activating the ERK1/2 signaling pathway, which positively regulated the NF-κB signaling pathway and negatively regulated the PI3K/AKT signaling pathway.
Rosamultin protected vascular endothelial cells against hypoxia-induced apoptosis by activating the PI3K/AKT signaling pathway and positively regulating ERK1/2 and NF-κB signaling pathways.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shi, Chaofeng& Zhan, Li& Wu, Yuqiang& Li, Zhengchao& Li, Jianyu& Li, Yaxiao…[et al.]. 2020. Kaji-Ichigoside F1 and Rosamultin Protect Vascular Endothelial Cells against Hypoxia-Induced Apoptosis via the PI3KAKT or ERK12 Signaling Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1205223
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shi, Chaofeng…[et al.]. Kaji-Ichigoside F1 and Rosamultin Protect Vascular Endothelial Cells against Hypoxia-Induced Apoptosis via the PI3KAKT or ERK12 Signaling Pathway. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1205223
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shi, Chaofeng& Zhan, Li& Wu, Yuqiang& Li, Zhengchao& Li, Jianyu& Li, Yaxiao…[et al.]. Kaji-Ichigoside F1 and Rosamultin Protect Vascular Endothelial Cells against Hypoxia-Induced Apoptosis via the PI3KAKT or ERK12 Signaling Pathway. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1205223
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1205223
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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