Ischemic Postconditioning Alleviates Intestinal Ischemia-Reperfusion Injury by Enhancing Autophagy and Suppressing Oxidative Stress through the AktGSK-3βNrf2 Pathway in Mice
المؤلفون المشاركون
Liu, Huimin
Wu, Yang
Meng, Qing-Tao
Leng, Yan
Li, Wei
Chen, Rong
Zhang, Yun-yan
Lan, Jia-nan
Hou, Jia-bao
Sun, Qian
Sun, Tao
Zeng, Zi
Tang, Ling-Hua
Xia, Zhong-Yuan
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-03-16
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Aims.
Ischemic postconditioning (IPO) has a strong protective effect against intestinal ischemia-reperfusion (IIR) injury that is partly related to autophagy.
However, the precise mechanisms involved are unknown.
Methods.
C57BL/6J mice were subjected to unilateral IIR with or without IPO.
After 45 min ischemia and 120 min reperfusion, intestinal tissues and blood were collected for examination.
HE staining and Chiu’s score were used to evaluate pathologic injury.
We test markers of intestinal barrier function and oxidative stress.
Finally, we used WB to detect the expression of key proteins of autophagy and the Akt/GSK-3β/Nrf2 pathway.
Results.
IPO significantly attenuated IIR injury.
Expression levels of LC3 II/I, Beclin-1, and p62 were altered during IIR, indicating that IPO enhanced autophagy.
IPO also activated Akt, inhibited GSK-3β, induced Nrf2 nuclear translocation, and upregulated HO-1 and NQO1 expression, thus providing protective effects against IIR injury by suppressing oxidative stress.
Consistently, the beneficial effects of IPO were abolished by pretreatment with 3-methyladenine, SC66, and brusatol, potent inhibitors of autophagy, Akt, and Nrf2, respectively.
Conclusion.
Our study indicates that IPO can ameliorate IIR injury by evoking autophagy, activating Akt, inactivating GSK-3β, and activating Nrf2.
These findings may provide novel insights for the alleviation of IIR injury.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Rong& Zhang, Yun-yan& Lan, Jia-nan& Liu, Huimin& Li, Wei& Wu, Yang…[et al.]. 2020. Ischemic Postconditioning Alleviates Intestinal Ischemia-Reperfusion Injury by Enhancing Autophagy and Suppressing Oxidative Stress through the AktGSK-3βNrf2 Pathway in Mice. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1205242
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Rong…[et al.]. Ischemic Postconditioning Alleviates Intestinal Ischemia-Reperfusion Injury by Enhancing Autophagy and Suppressing Oxidative Stress through the AktGSK-3βNrf2 Pathway in Mice. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1205242
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Rong& Zhang, Yun-yan& Lan, Jia-nan& Liu, Huimin& Li, Wei& Wu, Yang…[et al.]. Ischemic Postconditioning Alleviates Intestinal Ischemia-Reperfusion Injury by Enhancing Autophagy and Suppressing Oxidative Stress through the AktGSK-3βNrf2 Pathway in Mice. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1205242
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1205242
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر