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Methane Ameliorates Lipopolysaccharide-Induced Acute Orchitis by Anti-inflammatory, Antioxidative, and Antiapoptotic Effects via Regulation of the PK2PKR1 Pathway
المؤلفون المشاركون
Zhao, Dong-bao
Sun, Aijun
Huang, Chao
Zhang, Wenbo
Zhang, Xi
Guo, Jinping
Ji, Ruijuan
Qiao, Liang
Sun, Xuejun
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-19
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Objective.
The present study is aimed at investigating the anti-inflammatory, antioxidative, and antiapoptotic effects of methane on lipopolysaccharide- (LPS-) induced acute orchitis and its potential mechanisms.
Methods.
Adult male rats were intraperitoneally (i.p.) injected with methane-rich saline (MS, 20 mL/kg) following LPS (5 mg/kg, i.p.).
The survival rate was assessed every 12 h until 72 h after LPS induction, and surviving rats were sacrificed for further detection.
The wet/dry (W/D) ratio was determined, and testicular damage was histologically assessed.
Inflammatory cytokines in the testes and serum, including interleukin-1β (IL-1β), IL-6, IL-10, and tumor necrosis factor-α (TNF-α), were measured using ELISA and RT-qPCR.
Oxidative stress was evaluated by the level of superoxide dismutase (SOD) and malondialdehyde (MDA).
Testicular apoptosis was detected via TUNEL staining.
The expression of prokineticin 2 (PK2)/prokineticin receptor 1 (PKR1) was also analyzed using RT-qPCR, western blotting, and immunohistochemistry.
Results.
It was found that methane significantly prolonged rat survival, decreased the W/D ratio, alleviated LPS-induced histological changes, and reduced apoptotic cells in the testes.
Additionally, methane suppressed and promoted the production of pro- and anti-inflammatory cytokines, respectively.
Furthermore, methane significantly increased SOD levels, decreased MDA levels, and decreased testicular expression of PK2 and PKR1.
Therefore, methane exerts therapeutic effects on acute orchitis and might be a new and convenient strategy for the treatment of inflammation-related testicular diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Huang, Chao& Zhang, Wenbo& Sun, Aijun& Zhang, Xi& Guo, Jinping& Ji, Ruijuan…[et al.]. 2020. Methane Ameliorates Lipopolysaccharide-Induced Acute Orchitis by Anti-inflammatory, Antioxidative, and Antiapoptotic Effects via Regulation of the PK2PKR1 Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1205275
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Huang, Chao…[et al.]. Methane Ameliorates Lipopolysaccharide-Induced Acute Orchitis by Anti-inflammatory, Antioxidative, and Antiapoptotic Effects via Regulation of the PK2PKR1 Pathway. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1205275
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Huang, Chao& Zhang, Wenbo& Sun, Aijun& Zhang, Xi& Guo, Jinping& Ji, Ruijuan…[et al.]. Methane Ameliorates Lipopolysaccharide-Induced Acute Orchitis by Anti-inflammatory, Antioxidative, and Antiapoptotic Effects via Regulation of the PK2PKR1 Pathway. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1205275
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1205275
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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