In Vitro Studies on the Immunomodulatory Effects of Pulicaria crispa Extract on Human THP-1 Monocytes
المؤلفون المشاركون
Alkahtani, Saad
Albrahim, Tarfa
Alnasser, Moonerah M.
Al-Anazi, Mashael R.
ALKahtani, Muneera D.
al-Qahtani, Ahmed A.
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-09-26
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Background.
Pulicaria crispa (P.
crispa) is a plant from the Compositae family that exhibits antioxidant, anti-inflammatory, antibacterial, and cytotoxic activities.
Objective.
The current study aimed at investigating the immunomodulatory effects of P.
crispa extract in lipopolysaccharide- (LPS-) stimulated human monocytic THP-1 cells.
Methods.
To induce macrophage differentiation, THP-1 cell lines were treated with phorbol-12-myristate 13-acetate, followed by exposure to LPS with or without 50 or 100 μg/ml of P.
crispa extract.
The following tests were employed to test the immunomodulatory effects of the extract: MTT assay, ELISA, Western blotting analysis, cell migration and phagocytosis assays, and Annexin V staining method.
Results.
Exposure to 100 μg/ml P.
crispa extract significantly reduced THP-1 cell proliferation, migration, and phagocytosis (in LPS-stimulated cells, but not in unstimulated cells).
Moreover, the extract alone significantly reduced the rate of THP-1 cell apoptosis, while it increased the rate of late apoptosis.
Molecular investigations showed that treatment with P.
crispa extract significantly upregulated the expression of ERK1, p-MAPK, P-P38, and Bcl2, while it significantly reduced the expression of ERK5, Bax, NF-κB, P-NF-κB, CCL1, CCL2, CCL5, CCL22, CXCL1, and CXCL10.
Conclusion.
Pulicaria crispa extract exhibited anti-inflammatory, antiproliferative, antimigratory, and antiphagocytic effects in LPS-stimulated THP-1 cells.
Future studies should investigate these mechanisms in animal models with chronic inflammatory diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Albrahim, Tarfa& Alnasser, Moonerah M.& Al-Anazi, Mashael R.& ALKahtani, Muneera D.& Alkahtani, Saad& al-Qahtani, Ahmed A.. 2020. In Vitro Studies on the Immunomodulatory Effects of Pulicaria crispa Extract on Human THP-1 Monocytes. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1205396
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Albrahim, Tarfa…[et al.]. In Vitro Studies on the Immunomodulatory Effects of Pulicaria crispa Extract on Human THP-1 Monocytes. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1205396
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Albrahim, Tarfa& Alnasser, Moonerah M.& Al-Anazi, Mashael R.& ALKahtani, Muneera D.& Alkahtani, Saad& al-Qahtani, Ahmed A.. In Vitro Studies on the Immunomodulatory Effects of Pulicaria crispa Extract on Human THP-1 Monocytes. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1205396
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1205396
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر