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In Vitro Studies on the Immunomodulatory Effects of Pulicaria crispa Extract on Human THP-1 Monocytes
المؤلفون المشاركون
Alkahtani, Saad
Albrahim, Tarfa
Alnasser, Moonerah M.
Al-Anazi, Mashael R.
ALKahtani, Muneera D.
al-Qahtani, Ahmed A.
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-09-26
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Background.
Pulicaria crispa (P.
crispa) is a plant from the Compositae family that exhibits antioxidant, anti-inflammatory, antibacterial, and cytotoxic activities.
Objective.
The current study aimed at investigating the immunomodulatory effects of P.
crispa extract in lipopolysaccharide- (LPS-) stimulated human monocytic THP-1 cells.
Methods.
To induce macrophage differentiation, THP-1 cell lines were treated with phorbol-12-myristate 13-acetate, followed by exposure to LPS with or without 50 or 100 μg/ml of P.
crispa extract.
The following tests were employed to test the immunomodulatory effects of the extract: MTT assay, ELISA, Western blotting analysis, cell migration and phagocytosis assays, and Annexin V staining method.
Results.
Exposure to 100 μg/ml P.
crispa extract significantly reduced THP-1 cell proliferation, migration, and phagocytosis (in LPS-stimulated cells, but not in unstimulated cells).
Moreover, the extract alone significantly reduced the rate of THP-1 cell apoptosis, while it increased the rate of late apoptosis.
Molecular investigations showed that treatment with P.
crispa extract significantly upregulated the expression of ERK1, p-MAPK, P-P38, and Bcl2, while it significantly reduced the expression of ERK5, Bax, NF-κB, P-NF-κB, CCL1, CCL2, CCL5, CCL22, CXCL1, and CXCL10.
Conclusion.
Pulicaria crispa extract exhibited anti-inflammatory, antiproliferative, antimigratory, and antiphagocytic effects in LPS-stimulated THP-1 cells.
Future studies should investigate these mechanisms in animal models with chronic inflammatory diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Albrahim, Tarfa& Alnasser, Moonerah M.& Al-Anazi, Mashael R.& ALKahtani, Muneera D.& Alkahtani, Saad& al-Qahtani, Ahmed A.. 2020. In Vitro Studies on the Immunomodulatory Effects of Pulicaria crispa Extract on Human THP-1 Monocytes. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1205396
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Albrahim, Tarfa…[et al.]. In Vitro Studies on the Immunomodulatory Effects of Pulicaria crispa Extract on Human THP-1 Monocytes. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1205396
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Albrahim, Tarfa& Alnasser, Moonerah M.& Al-Anazi, Mashael R.& ALKahtani, Muneera D.& Alkahtani, Saad& al-Qahtani, Ahmed A.. In Vitro Studies on the Immunomodulatory Effects of Pulicaria crispa Extract on Human THP-1 Monocytes. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1205396
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1205396
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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