Cardiomyocyte Contractility and Autophagy in a Premature Senescence Model of Cardiac Aging
المؤلفون المشاركون
Grune, Tilman
Deubel, Stefanie
Ott, Christiane
Häseli, Steffen
Jung, Tobias
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-04-15
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Globally, cardiovascular diseases are the leading cause of death in the aging population.
While the clinical pathology of the aging heart is thoroughly characterized, underlying molecular mechanisms are still insufficiently clarified.
The aim of the present study was to establish an in vitro model system of cardiomyocyte premature senescence, culturing heart muscle cells derived from neonatal C57Bl/6J mice for 21 days.
Premature senescence of neonatal cardiac myocytes was induced by prolonged culture time in an oxygen-rich postnatal environment.
Age-related changes in cellular function were determined by senescence-associated β-galactosidase activity, increasing presence of cell cycle regulators, such as p16, p53, and p21, accumulation of protein aggregates, and restricted proteolysis in terms of decreasing (macro-)autophagy.
Furthermore, the culture system was functionally characterized for alterations in cell morphology and contractility.
An increase in cellular size associated with induced expression of atrial natriuretic peptides demonstrated a stress-induced hypertrophic phenotype in neonatal cardiomyocytes.
Using the recently developed analytical software tool Myocyter, we were able to show a spatiotemporal constraint in spontaneous contraction behavior during cultivation.
Within the present study, the 21-day culture of neonatal cardiomyocytes was defined as a functional model system of premature cardiac senescence to study age-related changes in cardiomyocyte contractility and autophagy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Häseli, Steffen& Deubel, Stefanie& Jung, Tobias& Grune, Tilman& Ott, Christiane. 2020. Cardiomyocyte Contractility and Autophagy in a Premature Senescence Model of Cardiac Aging. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1205522
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Häseli, Steffen…[et al.]. Cardiomyocyte Contractility and Autophagy in a Premature Senescence Model of Cardiac Aging. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1205522
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Häseli, Steffen& Deubel, Stefanie& Jung, Tobias& Grune, Tilman& Ott, Christiane. Cardiomyocyte Contractility and Autophagy in a Premature Senescence Model of Cardiac Aging. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1205522
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1205522
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر