Autophagy Functions to Prevent Methylglyoxal-Induced Apoptosis in HK-2 Cells
المؤلفون المشاركون
Ha, Tae Youl
Kim, Yoonsook
Jang, Young Jin
Park, So-Hyun
Choi, Hyun-Il
Seo, Hyo-Deok
Lee, Dae-Hee
Ahn, Jiyun
Jung, Chang Hwa
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-04
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Methylglyoxal (MGO), a reactive carbonyl species, causes cellular damage and is closely related to kidney disease, particularly diabetic nephropathy.
Although MGO has been reported to induce autophagy and apoptosis, the relationships between the two pathways are unclear.
Here, we evaluated whether autophagy may be the underlying mechanism inhibiting MGO-induced apoptosis.
MGO treatment induced concentration- and time-dependent apoptosis in HK-2 cells.
Moreover, MGO upregulated the autophagy markers p62 and LC3-II.
Apoptosis caused by MGO was increased in ATG5-knockdown cells compared to that in wild-type cells.
In contrast, autophagy activation by 5-aminoimidazole-4-carboxamide ribonucleotide resulted in reduced apoptosis, suggesting that autophagy played a role in protecting against MGO-induced cell death.
To examine the mechanisms through which autophagy occurred following MGO stimulation, we investigated changes in AKT/mammalian target of rapamycin (mTOR) signaling.
Autophagy induction by MGO treatment was not related to AKT/mTOR signaling; however, it did involve autophagy-related gene expression promoted by AMP-activated protein kinase-mediated transcription factors, such as forkhead box 1.
Overall, our findings indicate that MGO-induced cellular damage can be mitigated by autophagy, suggesting that autophagy may be a potential therapeutic target for diseases such as diabetic nephropathy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Park, So-Hyun& Choi, Hyun-Il& Ahn, Jiyun& Jang, Young Jin& Ha, Tae Youl& Seo, Hyo-Deok…[et al.]. 2020. Autophagy Functions to Prevent Methylglyoxal-Induced Apoptosis in HK-2 Cells. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1205574
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Park, So-Hyun…[et al.]. Autophagy Functions to Prevent Methylglyoxal-Induced Apoptosis in HK-2 Cells. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1205574
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Park, So-Hyun& Choi, Hyun-Il& Ahn, Jiyun& Jang, Young Jin& Ha, Tae Youl& Seo, Hyo-Deok…[et al.]. Autophagy Functions to Prevent Methylglyoxal-Induced Apoptosis in HK-2 Cells. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1205574
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1205574
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر