Proanthocyanidins Antagonize Arsenic-Induced Oxidative Damage and Promote Arsenic Methylation through Activation of the Nrf2 Signaling Pathway
المؤلفون المشاركون
Li, Shugang
Xu, Meng-Chuan
Niu, Qiang
Hu, Yun-Hua
Wang, Hai-Xia
Feng, Gangling
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-19، 19ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-01-20
دولة النشر
مصر
عدد الصفحات
19
التخصصات الرئيسية
الملخص EN
Purpose.
To investigate the effects of grape seed proanthocyanidin extract (GSPE) on oxidative damage and arsenic (As) methylation and to clarify the role of Nrf2 in the process.
Methods.
L-02 cells were treated with arsenic (25 μM) and GSPE (10, 25, and 50 mg/L) for 24 h.
Cell viability was analyzed by MTT assay.
Cell apoptosis and ROS fluorescence were detected by flow cytometry.
Oxidative stress marker levels were measured using commercial kits.
mRNA and protein expression were detected by qRT-PCR and western blotting.
The cellular concentrations of methylation products were measured by HPLC-HGAFS.
Arsenic methylation ability of cells was determined.
Results.
Cell survival rate was significantly lower in the As group than in the control group (P<0.05), while cell apoptosis increased and the number of apoptotic cells decreased gradually after GSPE intervention.
Superoxide dismutase, glutathione, and sulfhydryl levels in the intervention group were significantly higher (P<0.05), while MDA and ROS levels were significantly lower (P<0.05) than those in the As group.
The mRNA and protein expression of Nrf2, HO-1, NQO1, and glutathione-S-transferase increased in the As + GSPE group compared with that in the As group (P<0.05).
GSPE significantly increased methylated As level, primary methylation index, secondary methylation index, average growth rate of methylation, and average methylation speed compared with the GSPE untreated group (P<0.05).
After Nrf2 inhibition, the effect of GSPE decreased significantly.
Conclusion.
GSPE activates the Nrf2 signaling pathway to antagonize As-induced oxidative damage and to promote As methylation metabolism.
Therefore, GSPE may be a potential agent for relieving As-induced hepatotoxicity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xu, Meng-Chuan& Niu, Qiang& Hu, Yun-Hua& Feng, Gangling& Wang, Hai-Xia& Li, Shugang. 2019. Proanthocyanidins Antagonize Arsenic-Induced Oxidative Damage and Promote Arsenic Methylation through Activation of the Nrf2 Signaling Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-19.
https://search.emarefa.net/detail/BIM-1205754
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xu, Meng-Chuan…[et al.]. Proanthocyanidins Antagonize Arsenic-Induced Oxidative Damage and Promote Arsenic Methylation through Activation of the Nrf2 Signaling Pathway. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-19.
https://search.emarefa.net/detail/BIM-1205754
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xu, Meng-Chuan& Niu, Qiang& Hu, Yun-Hua& Feng, Gangling& Wang, Hai-Xia& Li, Shugang. Proanthocyanidins Antagonize Arsenic-Induced Oxidative Damage and Promote Arsenic Methylation through Activation of the Nrf2 Signaling Pathway. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-19.
https://search.emarefa.net/detail/BIM-1205754
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1205754
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر