Saikosaponin A-Induced Gut Microbiota Changes Attenuate Severe Acute Pancreatitis through the Activation of Keap1Nrf2-ARE Antioxidant Signaling

المؤلفون المشاركون

Li, Jing
Han, Jinfeng
Lv, Juan
Wang, Shiji
Qu, Lai
Jiang, Yanfang

المصدر

Oxidative Medicine and Cellular Longevity

العدد

المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-19، 19ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2020-11-02

دولة النشر

مصر

عدد الصفحات

19

التخصصات الرئيسية

الأحياء

الملخص EN

Objective.

Severe acute pancreatitis (SAP) is a serious and life-threatening disease associated with multiple organ failure and a high mortality rate and is accompanied by distinct oxidative stress and inflammatory responses.

Saikosaponin A has strong antioxidant properties and can affect the composition of gut microbiota.

We sought to determine the effects of Saikosaponin A interventions on SAP by investigating the changes of gut microbiota and related antioxidant signaling.

Methods.

A SAP model was established in Sprague-Dawley (SD) rats through the injection of sodium taurocholate into the biliopancreatic duct and confirmed by elevated levels of serum lipase and amylase.

The model was fed a standard diet either with saline solution or with Saikosaponin A.

Fecal microbiota transplantation (FMT) from Saikosaponin A-induced rats into the rat model was performed to test the effects of gut microbiota.

The composition of gut microbiota was analyzed by using 16S rRNA gene sequencing.

We measured apoptotic status, inflammatory biomarkers, and Keap1-Nrf2-ARE ((Kelch-like ECH-associated protein 1) nuclear factor erythroid 2-related factor 2-antioxidant response element) antioxidant signaling.

Results.

Saikosaponin A intervention attenuated SAP lesions and reduced the levels of serum amylase and lipase, oxidative stress, and inflammatory responses by reducing pathological scores and affecting the serum level of oxidative and inflammatory factors.

Meanwhile, the expression of Keap1-Nrf2-ARE was increased.

Saikosaponin A intervention improved microbiota composition by increasing the relative abundance of Lactobacillus and Prevotella species.

FMT resulted in similar results as those caused by the Saikosaponin A intervention, suggesting Saikosaponin A may exert its function via the improvement of gut microbiota composition.

Conclusions.

Saikosaponin A-induced gut microbiota changes attenuate SAP progression in the rat model and may be a potential natural drug for adjuvant treatment of SAP.

Further work is needed to clear up the points.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Li, Jing& Han, Jinfeng& Lv, Juan& Wang, Shiji& Qu, Lai& Jiang, Yanfang. 2020. Saikosaponin A-Induced Gut Microbiota Changes Attenuate Severe Acute Pancreatitis through the Activation of Keap1Nrf2-ARE Antioxidant Signaling. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-19.
https://search.emarefa.net/detail/BIM-1205982

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Li, Jing…[et al.]. Saikosaponin A-Induced Gut Microbiota Changes Attenuate Severe Acute Pancreatitis through the Activation of Keap1Nrf2-ARE Antioxidant Signaling. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-19.
https://search.emarefa.net/detail/BIM-1205982

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Li, Jing& Han, Jinfeng& Lv, Juan& Wang, Shiji& Qu, Lai& Jiang, Yanfang. Saikosaponin A-Induced Gut Microbiota Changes Attenuate Severe Acute Pancreatitis through the Activation of Keap1Nrf2-ARE Antioxidant Signaling. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-19.
https://search.emarefa.net/detail/BIM-1205982

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1205982