Antioxidant Effect of the Castanea sativa Mill. Leaf Extract on Oxidative Stress Induced upon Human Spermatozoa

المؤلفون المشاركون

Moretti, E.
Biagi, Marco
Noto, Daria
Cerretani, Daniela
Corsini, Maddalena
Baini, Giulia
Cappellucci, Giorgio

المصدر

Oxidative Medicine and Cellular Longevity

العدد

المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-9، 9ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2019-12-19

دولة النشر

مصر

عدد الصفحات

9

التخصصات الرئيسية

الأحياء

الملخص EN

This study was aimed at evaluating in vitro the effects of a 75% v/v ethanolic extract of leaves of Castanea sativa Mill.

(var.

Bastarda Rossa, Mount Amiata, Tuscany, Italy) on ejaculated human sperm.

Total polyphenols and total flavonoids contained in the extract were determined by a colorimetric assay and HPLC-DAD.

The DPPH test and electrochemistry were utilized to study the antioxidant activity of the extract.

Swim-up-selected sperm from 8 healthy men were treated with the C.

sativa leaf extract at different dilutions (1 : 100, 1 : 200, and 1 : 500), and sperm motility was assessed following the WHO guidelines.

Swim-up-selected spermatozoa were incubated with 100 μM H2O2 to induce lipid peroxidation (LPO) and with H2O2 and the leaf extract (1 : 100, 1 : 200, and 1 : 500) to test the antioxidant activity of the extract.

The levels of LPO were determined by measuring malondialdehyde (MDA) concentrations.

The treated samples were also analyzed by transmission electron microscopy (TEM) for ultrastructural evaluation.

The chemical analysis showed that one-third ca.

of the polyphenols in the C.

sativa extract were made up of flavonoids, with hyperoside present in high concentration.

A good antioxidant activity was demonstrated by both the DPPH test and electrochemical analysis.

The C.

sativa leaf extract did not decrease sperm motility at all tested dilutions.

Treatment with H2O2 alone caused a significant increment in MDA levels (P=0.006993), while the treatment with H2O2 plus C.

sativa extract diluted to 1 : 100 and 1 : 200 significantly reduced MDA levels (P=0.01476 and P=0.01571, respectively), with respect to H2O2 alone.

TEM analysis confirmed the protective effect of the extract on damage induced by LPO, in particular that occurring at the plasma membrane level.

The C.

sativa leaf extract could be used in human and farm animal protocols for gamete handling, such as techniques of assisted reproduction and cryopreservation of semen, all conditions in which oxidative stress is exacerbated.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Biagi, Marco& Noto, Daria& Corsini, Maddalena& Baini, Giulia& Cerretani, Daniela& Cappellucci, Giorgio…[et al.]. 2019. Antioxidant Effect of the Castanea sativa Mill. Leaf Extract on Oxidative Stress Induced upon Human Spermatozoa. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1205988

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Biagi, Marco…[et al.]. Antioxidant Effect of the Castanea sativa Mill. Leaf Extract on Oxidative Stress Induced upon Human Spermatozoa. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1205988

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Biagi, Marco& Noto, Daria& Corsini, Maddalena& Baini, Giulia& Cerretani, Daniela& Cappellucci, Giorgio…[et al.]. Antioxidant Effect of the Castanea sativa Mill. Leaf Extract on Oxidative Stress Induced upon Human Spermatozoa. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1205988

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1205988