ZNRD1 and Its Antisense Long Noncoding RNA ZNRD1-AS1 Are Oppositely Regulated by Cold Atmospheric Plasma in Breast Cancer Cells
المؤلفون المشاركون
Choi, Eun Ha
Kim, Hyeon Woo
Jeong, Dawoon
Ham, Juyeon
Kim, Heejoo
Ji, Hwee Won
Kim, Sun Jung
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-05-06
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Cold atmospheric plasma (CAP) has been recognized as a potential alternative or supplementary cancer treatment tool, which is attributed by its selective antiproliferation effect on cancer cells over normal cells.
Standardization of the CAP treatment in terms of biological outputs such as cell growth inhibition and gene expression change is essential for its clinical application.
This study aims at identifying genes that show consistent expression profiles at a specific CAP condition, which could be used to monitor whether CAP is an appropriate treatment to biological targets.
To do this, genes showing differential expression by two different CAP treatment conditions were screened in the MCF-7 breast cancer cells.
As a result, ZNRD1 was identified as a potential marker with being consistently upregulated by 600 s but downregulated by the 10×30 s CAP treatment scheme.
Expression of ZNRD1 was increased in breast cancer tissues compared to normal tissues, judged by cancer tissue database analysis, and supported by the antiproliferation after siRNA-induced downregulation in MCF-7.
Interestingly, the antisense long noncoding RNA (lncRNA) of ZNRD1, ZNRD1-AS1, was regulated to the opposite direction of ZNRD1 by CAP.
The siRNA-based qPCR analysis indicates that ZNRD1 downregulates ZNRD1-AS1, but not vice versa.
ZNRD1-AS1 was shown to increase a few cis-genes such as HLA-A, HCG9, and PPP1R11 that were also regulated by CAP.
Altogether, this study identified a pair of gene and its antisense lncRNA of which expression is precisely controlled by CAP in a dose-dependent manner.
These genes could help elucidate the molecular mechanism how CAP regulates lncRNAs in cancer cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kim, Hyeon Woo& Jeong, Dawoon& Ham, Juyeon& Kim, Heejoo& Ji, Hwee Won& Choi, Eun Ha…[et al.]. 2020. ZNRD1 and Its Antisense Long Noncoding RNA ZNRD1-AS1 Are Oppositely Regulated by Cold Atmospheric Plasma in Breast Cancer Cells. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1206060
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kim, Hyeon Woo…[et al.]. ZNRD1 and Its Antisense Long Noncoding RNA ZNRD1-AS1 Are Oppositely Regulated by Cold Atmospheric Plasma in Breast Cancer Cells. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1206060
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kim, Hyeon Woo& Jeong, Dawoon& Ham, Juyeon& Kim, Heejoo& Ji, Hwee Won& Choi, Eun Ha…[et al.]. ZNRD1 and Its Antisense Long Noncoding RNA ZNRD1-AS1 Are Oppositely Regulated by Cold Atmospheric Plasma in Breast Cancer Cells. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1206060
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1206060
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر