Effects of the Insulted Neuronal Cells-Derived Extracellular Vesicles on the Survival of Umbilical Cord-Derived Mesenchymal Stem Cells following Cerebral IschemiaReperfusion Injury
المؤلفون المشاركون
Huang, Yan
Tan, Fengbo
Liu, Zuo
Lu, Ming
Hu, Zhiping
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-26، 26ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-07-17
دولة النشر
مصر
عدد الصفحات
26
التخصصات الرئيسية
الملخص EN
Umbilical cord-derived mesenchymal stem cells (UC-MSCs) engraftment is a potential therapy for cerebral ischemic stroke.
However, the harsh microenvironment induced by cerebral ischemia/reperfusion restricts the survival rate and therapeutic efficiency of the engrafted UC-MSCs.
In this study, we explored whether small extracellular vesicles (EVs) derived from injured neuronal cells following exposure to cerebral ischemia/reperfusion insult affect the survival of transplanted UC-MSCs.
To establish a simulation of cerebral ischemia/reperfusion microenvironment comprising engrafted UC-MSCs and neuronal cells, we cocultured EVs derived from injured N2A cells, caused by exposure to oxygen-glucose deprivation and reperfusion (OGD/R) insult, with UC-MSCs in a conditioned medium.
Coculture of UC-MSCs with EVs exacerbated the OGD/R-induced apoptosis and oxidative stress.
Suppression of EVs-release via knock-down of Rab27a effectively protected the UC-MSCs from OGD/R-induced insult.
Moreover, hypoxia preconditioning not only elevated the survival of UC-MSCs but also improved the paracrine mechanism of injured N2A cells.
Altogether, these results show that EVs from injured N2A cells exacerbates OGD/R-induced injury on transplanted UC-MSCs in vitro.
Hypoxia preconditioning enhances the survival of the engrafted-UC-MSCs; hence, thus could be an effective approach for improving UC-MSCs therapy in ischemic stroke.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Huang, Yan& Liu, Zuo& Tan, Fengbo& Hu, Zhiping& Lu, Ming. 2020. Effects of the Insulted Neuronal Cells-Derived Extracellular Vesicles on the Survival of Umbilical Cord-Derived Mesenchymal Stem Cells following Cerebral IschemiaReperfusion Injury. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-26.
https://search.emarefa.net/detail/BIM-1206148
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Huang, Yan…[et al.]. Effects of the Insulted Neuronal Cells-Derived Extracellular Vesicles on the Survival of Umbilical Cord-Derived Mesenchymal Stem Cells following Cerebral IschemiaReperfusion Injury. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-26.
https://search.emarefa.net/detail/BIM-1206148
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Huang, Yan& Liu, Zuo& Tan, Fengbo& Hu, Zhiping& Lu, Ming. Effects of the Insulted Neuronal Cells-Derived Extracellular Vesicles on the Survival of Umbilical Cord-Derived Mesenchymal Stem Cells following Cerebral IschemiaReperfusion Injury. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-26.
https://search.emarefa.net/detail/BIM-1206148
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1206148
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر