Somatic Mitochondrial DNA Point Mutations Used as Biomarkers to Demonstrate Genomic Heterogeneity in Primary Prostate Cancer
المؤلفون المشاركون
Arstad, Christian
Taskén, Kristin
Refinetti, Paulo
Axcrona, Ulrika
Giercksky, Karl-Erik
Ekstrøm, Per Olaf
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-28
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Primary prostate tumor heterogeneity is poorly understood, leaving research efforts with challenges regarding the initiation and advancement of the disease.
The growth of tumor cells is accompanied by mutations in nuclear and in mitochondrial genomes.
Thus, mitochondrial DNA mutations may be used as tumor cell markers.
By the use of laser capture microdissection coupled with assays for mitochondrial point mutation detection, mtDNA mutations were used to trace mutated cells at a histological level.
Point mutations in mtDNA were determined in 12 primary prostate cancers.
The tumors represent different pathology-prognostic grade groups.
Known mutational hotspots of the mtDNA were scanned for heteroplasmy.
All specimens with mtDNA heteroplasmy were subsequently subsampled by laser capture microdissection.
From a total number of 1728 microsamples, mitochondrial DNA target sequences were amplified and base substitutions detected by cycling temperature capillary electrophoresis.
Real-time PCR was used as a quantitative assay to determine the relative mtDNA copy number of 12 tumors studied, represented by two samples from each (N = 24); a high degree (75%) demonstrated tumor specimen heterogeneity.
A grid of 96 spots isolated by laser capture microdissection demonstrated interfocal sample heterogeneity and increased the limit of detection.
The spots demonstrated a wide range of mutant fractions from 0 to 100% mutant copies.
The mitochondrial DNA copy number in the samples was determined by real-time PCR.
No correlation between copy number and pathology-prognostic grade groups was observed.
Somatic mitochondrial DNA point mutations represent traceable biomarkers demonstrating heterogeneity in primary prostate cancer.
Mutations can be detected in areas before changes in tissue histopathology are evident to the pathologist.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Arstad, Christian& Taskén, Kristin& Refinetti, Paulo& Axcrona, Ulrika& Giercksky, Karl-Erik& Ekstrøm, Per Olaf. 2020. Somatic Mitochondrial DNA Point Mutations Used as Biomarkers to Demonstrate Genomic Heterogeneity in Primary Prostate Cancer. Prostate Cancer،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1206355
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Arstad, Christian…[et al.]. Somatic Mitochondrial DNA Point Mutations Used as Biomarkers to Demonstrate Genomic Heterogeneity in Primary Prostate Cancer. Prostate Cancer No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1206355
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Arstad, Christian& Taskén, Kristin& Refinetti, Paulo& Axcrona, Ulrika& Giercksky, Karl-Erik& Ekstrøm, Per Olaf. Somatic Mitochondrial DNA Point Mutations Used as Biomarkers to Demonstrate Genomic Heterogeneity in Primary Prostate Cancer. Prostate Cancer. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1206355
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1206355
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر