Melatonin Prevents Osteoarthritis-Induced Cartilage Degradation via Targeting MicroRNA-140

المؤلفون المشاركون

He, Fan
Yang, Huilin
Ming, Pei
Zhang, Yijian
Lin, Jun
Zhou, Xinfeng
Chen, Xi
Chen, Angela Carley
Pi, Bin
Pan, Guoqing
Liu, Tao

المصدر

Oxidative Medicine and Cellular Longevity

العدد

المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-16، 16ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2019-12-14

دولة النشر

مصر

عدد الصفحات

16

التخصصات الرئيسية

الأحياء

الملخص EN

Osteoarthritis (OA) is characterized by the progressive destruction of articular cartilage, which is involved in the imbalance between extracellular matrix (ECM) synthesis and degradation.

MicroRNA-140-5p (miR-140) is specifically expressed in cartilage and plays an important role in OA-induced matrix degradation.

The aim of this study was to investigate (1) whether intra-articular injection of melatonin could ameliorate surgically induced OA in mice and (2) whether melatonin could regulate matrix-degrading enzymes at the posttranscriptional level by targeting miR-140.

In an in vitro OA environment induced by interleukin-1 beta (IL-1β), melatonin treatment improved cell proliferation of human chondrocytes, promoted the expression of cartilage ECM proteins (e.g., type II collagen and aggrecan), and inhibited the levels of IL-1β-induced proteinases, such as matrix metalloproteinase 9 (MMP9), MMP13, ADAMTS4 (a disintegrin and metalloproteinase with thrombospondin motifs 4), and ADAMTS5.

Both the microarray and polymerase chain reaction (PCR) experiments revealed that miR-140 was a melatonin-responsive microRNA and melatonin upregulated miR-140 expression, which was suppressed by IL-1β stimulation.

In vivo experiments demonstrated that intra-articular injection of melatonin prevented disruptions of cartilage matrix homeostasis and successfully alleviated the progression of surgery-induced OA in mice.

Transfection of miR-140 antagomir completely counteracted the antiarthritic effects of melatonin by promoting matrix destruction.

Our findings demonstrate that melatonin protects the articular cartilage from OA-induced degradation by targeting miR-140, and intra-articular administration of melatonin may benefit patients suffering from OA.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Zhang, Yijian& Lin, Jun& Zhou, Xinfeng& Chen, Xi& Chen, Angela Carley& Pi, Bin…[et al.]. 2019. Melatonin Prevents Osteoarthritis-Induced Cartilage Degradation via Targeting MicroRNA-140. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1206572

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Zhang, Yijian…[et al.]. Melatonin Prevents Osteoarthritis-Induced Cartilage Degradation via Targeting MicroRNA-140. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1206572

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Zhang, Yijian& Lin, Jun& Zhou, Xinfeng& Chen, Xi& Chen, Angela Carley& Pi, Bin…[et al.]. Melatonin Prevents Osteoarthritis-Induced Cartilage Degradation via Targeting MicroRNA-140. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1206572

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1206572