Elevated Intraocular Pressure Causes Abnormal Reactivity of Mouse Retinal Arterioles
المؤلفون المشاركون
Daiber, Andreas
Prokosch, V.
Pfeiffer, Norbert
Gericke, Adrian
Zadeh, Jenia Kouchek
Musayeva, Aytan
Xia, Ning
Li, Huige
Mann, Carolina
Wolff, Ismael
Wang, Maoren
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-30
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Objective.
Glaucoma is a leading cause of severe visual impairment and blindness.
Although high intraocular pressure (IOP) is an established risk factor for the disease, the role of abnormal ocular vessel function in the pathophysiology of glaucoma gains more and more attention.
We tested the hypothesis that elevated intraocular pressure (IOP) causes vascular dysfunction in the retina.
Methods.
High IOP was induced in one group of mice by unilateral cauterization of three episcleral veins.
The other group received sham surgery only.
Two weeks later, retinal vascular preparations were studied by video microscopy in vitro.
Reactive oxygen species (ROS) levels and expression of hypoxia markers and of prooxidant and antioxidant redox genes as well as of inflammatory cytokines were determined.
Results.
Strikingly, responses of retinal arterioles to stepwise elevation of perfusion pressure were impaired in the high-IOP group.
Moreover, vasodilation responses to the endothelium-dependent vasodilator, acetylcholine, were markedly reduced in mice with elevated IOP, while no differences were seen in response to the endothelium-independent nitric oxide donor, sodium nitroprusside.
Remarkably, ROS levels were increased in the retinal ganglion cell layer including blood vessels.
Expression of the NADPH oxidase isoform, NOX2, and of the inflammatory cytokine, TNF-α, was increased at the mRNA level in retinal explants.
Expression of NOX2, but not of the hypoxic markers, HIF-1α and VEGF-A, was increased in the retinal ganglion cell layer and in retinal blood vessels at the protein level.
Conclusion.
Our data provide first-time evidence that IOP elevation impairs autoregulation and induces endothelial dysfunction in mouse retinal arterioles.
Oxidative stress and inflammation, but not hypoxia, appear to be involved in this process.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gericke, Adrian& Mann, Carolina& Zadeh, Jenia Kouchek& Musayeva, Aytan& Wolff, Ismael& Wang, Maoren…[et al.]. 2019. Elevated Intraocular Pressure Causes Abnormal Reactivity of Mouse Retinal Arterioles. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1206620
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gericke, Adrian…[et al.]. Elevated Intraocular Pressure Causes Abnormal Reactivity of Mouse Retinal Arterioles. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1206620
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gericke, Adrian& Mann, Carolina& Zadeh, Jenia Kouchek& Musayeva, Aytan& Wolff, Ismael& Wang, Maoren…[et al.]. Elevated Intraocular Pressure Causes Abnormal Reactivity of Mouse Retinal Arterioles. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1206620
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1206620
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر