Curcumin Protects Human Umbilical Vein Endothelial Cells against H2O2-Induced Cell Injury
المؤلفون المشاركون
Ouyang, Jipeng
Li, Rong
Shi, Haiqin
Zhong, Jianping
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-09-02
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Migraine is a prevalent neurological disorder which causes a huge economic burden on society.
It is thought to be a neurovascular disease with oxidative stress might be involved.
Curcumin, one of the major ingredients of turmeric, has potent antioxidative and anti-inflammatory properties, but whether it could be used as a potential treatment for migraine remains to be explored.
In the present study, human umbilical vein endothelial cells (HUVECs) were pretreated with various concentrations of curcumin (0 μM, 10 μM, 20 μM, 30 μM, 40 μM, and 50 μM) for 12 h, thereby exposed to H2O2 (100 μM) for another 12 h.
The viability of HUVECs was tested by the CCK-8 assay, and the activities of antioxidant enzymes including superoxide dismutase (SOD) and glutathione (GSH) were also examined.
Intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) were assayed to determine H2O2-induced oxidative stress.
In addition, several cell death-related genes (p53, p21, Bax, and Bcl-2) were detected by PCR, and an apoptosis-related protein (caspase3) was evaluated by western blotting.
Our results showed that curcumin improved the H2O2-induced decrease of cell viability and antioxidative enzyme activities and decreased the level of oxidative stress.
As a conclusion, curcumin could mitigate H2O2-induced oxidative stress and cell death in HUVECs and may be a potential therapeutic drug for migraine.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ouyang, Jipeng& Li, Rong& Shi, Haiqin& Zhong, Jianping. 2019. Curcumin Protects Human Umbilical Vein Endothelial Cells against H2O2-Induced Cell Injury. Pain Research and Management،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1207256
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ouyang, Jipeng…[et al.]. Curcumin Protects Human Umbilical Vein Endothelial Cells against H2O2-Induced Cell Injury. Pain Research and Management No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1207256
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ouyang, Jipeng& Li, Rong& Shi, Haiqin& Zhong, Jianping. Curcumin Protects Human Umbilical Vein Endothelial Cells against H2O2-Induced Cell Injury. Pain Research and Management. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1207256
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1207256
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر