5-Azacytidine-Induced Cardiomyocyte Differentiation of Very Small Embryonic-Like Stem Cells
المؤلفون المشاركون
Sun, XiaoLin
Li, HongXiao
Zhu, Ye
Xu, Pei
Zuo, QiSheng
Li, BiChun
Gu, Xiang
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-09-08
دولة النشر
مصر
عدد الصفحات
8
الملخص EN
The use of stem cells in generating cell-based pacemaker therapies for bradyarrhythmia is currently being considered.
Due to the propensity of stem cells to form tumors, as well as ethical issues surrounding their use, the seed cells used in cardiac biological pacemakers have limitations.
Very small embryonic-like stem cells (VSELs) are a unique and rare adult stem cell population, which have the same structural, genetic, biochemical, and functional characteristics as embryonic stem cells without the ethical controversy.
In this study, we investigated the ability of rat bone marrow- (BM-) derived VSELs to differentiate in vitro into cardiomyocytes by 5-Azacytidine (5-AzaC) treatment.
The morphology of VSELs treated with 10 μM 5-AzaC increased in volume and gradually changed to cardiomyocyte-like morphology without massive cell death.
Additionally, mRNA expression of the cardiomyocyte markers cardiac troponin-T (cTnT) and α-sarcomeric actin (α-actin) was significantly upregulated after 5-AzaC treatment.
Conversely, stem cell markers such as Nanog, Oct-4, and Sox2 were continuously downregulated posttreatment.
On day 14 post-5-AzaC treatment, the positive expression rates of cTnT and α-actin were 18.41±1.51% and 19.43±0.51%, respectively.
Taken together, our results showed that rat BM-VSELs have the ability to differentiate into cardiomyocytes in vitro.
These findings suggest that VSELs would be useful as seed cells in exploring the mechanism of biological pacemaker activity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sun, XiaoLin& Li, HongXiao& Zhu, Ye& Xu, Pei& Zuo, QiSheng& Li, BiChun…[et al.]. 2020. 5-Azacytidine-Induced Cardiomyocyte Differentiation of Very Small Embryonic-Like Stem Cells. Stem Cells International،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1207780
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sun, XiaoLin…[et al.]. 5-Azacytidine-Induced Cardiomyocyte Differentiation of Very Small Embryonic-Like Stem Cells. Stem Cells International No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1207780
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sun, XiaoLin& Li, HongXiao& Zhu, Ye& Xu, Pei& Zuo, QiSheng& Li, BiChun…[et al.]. 5-Azacytidine-Induced Cardiomyocyte Differentiation of Very Small Embryonic-Like Stem Cells. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1207780
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1207780
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر