Shear Stress Triggers Angiogenesis of Late Endothelial Progenitor Cells via the PTENAktGTPCHBH4 Pathway
المؤلفون المشاركون
Yao, Shun
Zeng, Haitao
Wu, Shaohong
Yang, Zhen
Zhu, Ling-Ping
Zhang, Feng
Tang, Lu
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-04-30
دولة النشر
مصر
عدد الصفحات
13
الملخص EN
Background.
Shear stress is an effective modulator of endothelial progenitor cells (EPCs) and has been suggested to play an important role in angiogenesis.
The phosphatase and tensin homolog (PTEN)/Akt and guanosine triphosphate cyclohydrolase (GTPCH)/tetrahydrobiopterin (BH4) pathways regulate the function of early EPCs.
However, the role of these pathways in the shear stress-induced angiogenesis of late EPCs remains poorly understood.
Therefore, we aim to investigate whether shear stress could upregulate the angiogenesis capacity of late EPCs and to further explore the possible underlying mechanisms.
Methods.
Late EPCs were subjected to laminar shear stress (LSS), and their in vitro migration, proliferation, and tube formation capacity were determined.
In addition, the in vivo angiogenesis capacity was explored, along with the expression of molecules involved in the PTEN/Akt and GTPCH/BH4 pathways.
Results.
LSS elevated the in vitro activities of late EPCs, which were accompanied by downregulated PTEN expression, accelerated Akt phosphorylation, and GTPCH/BH4 pathway activation (all P<0.05).
Following Akt inhibition, LSS-induced upregulated GTPCH expression, BH4, and NO level of EPCs were suppressed.
LSS significantly improved the migration, proliferation, and tube formation ability (15 dyn/cm2 LSS vs.
stationary: 72.2±5.5 vs.
47.3±7.3, 0.517±0.05 vs.
0.367±0.038, and 1.664±0.315 vs.
1±0, respectively; all P<0.05) along with the in vivo angiogenesis capacity of late EPCs, contributing to the recovery of limb ischemia.
These effects were also blocked by Akt inhibition or GTPCH knockdown (P<0.05, respectively).
Conclusions.
This study provides the first evidence that shear stress triggers angiogenesis in late EPCs via the PTEN/Akt/GTPCH/BH4 pathway, providing a potential nonpharmacologic therapeutic strategy for promoting angiogenesis in ischemia-related diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wu, Shaohong& Zhang, Feng& Yao, Shun& Tang, Lu& Zeng, Haitao& Zhu, Ling-Ping…[et al.]. 2020. Shear Stress Triggers Angiogenesis of Late Endothelial Progenitor Cells via the PTENAktGTPCHBH4 Pathway. Stem Cells International،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1207826
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wu, Shaohong…[et al.]. Shear Stress Triggers Angiogenesis of Late Endothelial Progenitor Cells via the PTENAktGTPCHBH4 Pathway. Stem Cells International No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1207826
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wu, Shaohong& Zhang, Feng& Yao, Shun& Tang, Lu& Zeng, Haitao& Zhu, Ling-Ping…[et al.]. Shear Stress Triggers Angiogenesis of Late Endothelial Progenitor Cells via the PTENAktGTPCHBH4 Pathway. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1207826
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1207826
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر