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c-Jun Overexpression Accelerates Wound Healing in Diabetic Rats by Human Umbilical Cord-Derived Mesenchymal Stem Cells
المؤلفون المشاركون
Wan, Xin-xing
Guo, Zi
Luo, Yufang
Yuan, Jingjing
Mo, Zhaohui
Yue, Chun
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-01-14
دولة النشر
مصر
عدد الصفحات
10
الملخص EN
Objective.
Mesenchymal stem cells (MSCs) are considered a promising therapy for wound healing.
Here, we explored the role of c-Jun in diabetic wound healing using human umbilical cord-derived MSCs (hUC-MSCs).
Methods.
Freshly isolated hUC-MSCs were subjected to extensive in vitro subcultivation.
The cell proliferative and migratory capacities were assessed by the Cell Counting Kit-8 and scratch assays, respectively.
c-Jun expression was evaluated by RT-PCR and western blot analysis.
The function of c-Jun was investigated with lentivirus transduction-based gene silencing and overexpression.
Diabetes mellitus was induced in SD rats on a high-glucose/fat diet by streptozocin administration.
Wounds were created on the dorsal skin.
The effects of c-Jun silencing and overexpression on wound closure by hUC-MSCs were examined.
Reepithelialization and angiogenesis were assessed by histological and immunohistochemical analysis, respectively.
Platelet-derived growth factor A (PDGFA), hepatocyte growth factor (HGF), and vascular endothelial growth factor (VEGF) levels were determined by western blot analysis.
Results.
hUC-MSCs showed gradually decreased cell proliferation, migration, and c-Jun expression during subcultivation.
c-Jun silencing inhibited cell proliferation and migration, while c-Jun overexpression enhanced proliferation but not migration.
Compared with untransduced hUC-MSCs, local subcutaneous injection of c-Jun-overexpressing hUC-MSCs accelerated wound closure, enhanced angiogenesis and reepithelialization at the wound bed, and increased PDGFA and HGF levels in wound tissues.
Conclusion.
c-Jun overexpression promoted hUC-MSC proliferation and migration in vitro and accelerated diabetic wound closure, reepithelization, and angiogenesis by hUC-MSCs in vivo.
These beneficial effects of c-Jun overexpression in diabetic wound healing by hUC-MSCs were at least partially mediated by increased PDGFA and HGF levels in wound tissues.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yue, Chun& Guo, Zi& Luo, Yufang& Yuan, Jingjing& Wan, Xin-xing& Mo, Zhaohui. 2020. c-Jun Overexpression Accelerates Wound Healing in Diabetic Rats by Human Umbilical Cord-Derived Mesenchymal Stem Cells. Stem Cells International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1207863
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yue, Chun…[et al.]. c-Jun Overexpression Accelerates Wound Healing in Diabetic Rats by Human Umbilical Cord-Derived Mesenchymal Stem Cells. Stem Cells International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1207863
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yue, Chun& Guo, Zi& Luo, Yufang& Yuan, Jingjing& Wan, Xin-xing& Mo, Zhaohui. c-Jun Overexpression Accelerates Wound Healing in Diabetic Rats by Human Umbilical Cord-Derived Mesenchymal Stem Cells. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1207863
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1207863
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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