Generation of Insulin-Producing Cells from Canine Adipose Tissue-Derived Mesenchymal Stem Cells
المؤلفون المشاركون
Teshima, Takahiro
Okamoto, Keiji
Dairaku, Kazuho
Nagashima, Tomokazu
Michishita, Masaki
Suzuki, Ryohei
Matsumoto, Hirotaka
Koyama, Hidekazu
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-10-19
دولة النشر
مصر
عدد الصفحات
10
الملخص EN
The potential of mesenchymal stem cells (MSCs) to differentiate into nonmesodermal cells such as pancreatic beta cells has been reported.
New cell-based therapy using MSCs for diabetes mellitus is anticipated as an alternative treatment option to insulin injection or islet transplantation in both human and veterinary medicine.
Several protocols were reported for differentiation of MSCs into insulin-producing cells (IPCs), but no studies have reported IPCs generated from canine MSCs.
The purpose of this study was to generate IPCs from canine adipose tissue-derived MSCs (AT-MSCs) in vitro and to investigate the effects of IPC transplantation on diabetic mice in vivo.
Culturing AT-MSCs with the differentiation protocol under a two-dimensional culture system did not produce IPCs.
However, spheroid-like small clusters consisting of canine AT-MSCs and human recombinant peptide μ-pieces developed under a three-dimensional (3D) culture system were successfully differentiated into IPCs.
The generated IPCs under 3D culture condition were stained with dithizone and anti-insulin antibody.
Canine IPCs also showed gene expression typical for pancreatic beta cells and increased insulin secretion in response to glucose stimulation.
The blood glucose levels in streptozotocin-induced diabetic mice were decreased after injection with the supernatant of canine IPCs, but the hyperglycemic states of diabetic mice were not improved after transplanting IPCs subcutaneously or intramesenterically.
The histological examination showed that the transplanted small clusters of IPCs were successfully engrafted to the mice and included cells positive for insulin by immunofluorescence.
Several factors, such as the transplanted cell number, the origin of AT-MSCs, and the differentiation protocol, were considered potential reasons for the inability to improve the hyperglycemic state after IPC transplantation.
These findings suggest that canine AT-MSCs can be differentiated into IPCs under a 3D culture system and IPC transplantation may be a new treatment option for dogs with diabetes mellitus.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Teshima, Takahiro& Okamoto, Keiji& Dairaku, Kazuho& Nagashima, Tomokazu& Michishita, Masaki& Suzuki, Ryohei…[et al.]. 2020. Generation of Insulin-Producing Cells from Canine Adipose Tissue-Derived Mesenchymal Stem Cells. Stem Cells International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1207956
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Teshima, Takahiro…[et al.]. Generation of Insulin-Producing Cells from Canine Adipose Tissue-Derived Mesenchymal Stem Cells. Stem Cells International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1207956
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Teshima, Takahiro& Okamoto, Keiji& Dairaku, Kazuho& Nagashima, Tomokazu& Michishita, Masaki& Suzuki, Ryohei…[et al.]. Generation of Insulin-Producing Cells from Canine Adipose Tissue-Derived Mesenchymal Stem Cells. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1207956
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1207956
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر