Transplantation Site Affects the Outcomes of Adipose-Derived Stem Cell-Based Therapy for Retinal Degeneration
المؤلفون المشاركون
Lu, Lixia
Tian, Haibin
Xu, Guo-Tong
Hu, Chengyu
La, Huanzhi
Wei, Xuancheng
Zhou, Yue
Ou, Qingjian
Chen, Zhiyang
Zhu, Xiaoman
Xu, Jing-Ying
Jin, Caixia
Gao, Furong
Wang, Juan
Zhang, Jingfa
Zhang, Jieping
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-01-06
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Adipose-derived stem cells (ASCs) have shown a strong protective effect on retinal degenerative diseases (RDD) after being transplanted into the subretinal space in an animal model.
Recently, several clinical trials have been conducted to treat RDD with intravitreal transplantation of stem cells, including ASCs.
However, the outcomes of the clinical trials were not satisfactory.
To investigate if the transplantation site alters the outcome of stem cell-based therapy for RDD, we isolated rat ASCs (rASCs) and labeled them with green fluorescent protein.
Autologous rASCs were grafted into the vitreous chamber or subretinal space in a rat RDD model induced by sodium iodate (SI).
The electric response was recorded by ERG.
The anatomic structure of the retina was observed in cryosections of rat eyes at posttransplantation weeks 1, 2, and 4.
Neural retina apoptosis and epiretinal membrane- (ERM-) like structure formation were investigated by immunostaining.
The intravitreal transplantation of rASCs resulted in an extinguished electric response, although the rosette formation and apoptosis of neural retina were reduced.
However, the rASCs that grafted in the subretinal space protected the retina from the damage caused by SI, including a partial recovering of the electric response and a reduction in rosette formation.
Intravitreally grafted rASCs formed a membrane, resulting in retina folding at the injection site.
Müller cells, retinal pigment epithelial cells, and microglial cells migrated from the retina to the rASC-formed membrane and subsequently formed an ERM-like structure.
Furthermore, vitreous fluid promoted rASC migration, and rASC-conditioned medium enhanced Müller cell migration as indicated by in vitro studies.
These data suggested that the vitreous chamber is not a good transplantation site for ASC-based therapy for RDD and that a deliberate decision should be made before transplantation of stem cells into the vitreous chamber to treat RDD in clinical trials.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hu, Chengyu& La, Huanzhi& Wei, Xuancheng& Zhou, Yue& Ou, Qingjian& Chen, Zhiyang…[et al.]. 2020. Transplantation Site Affects the Outcomes of Adipose-Derived Stem Cell-Based Therapy for Retinal Degeneration. Stem Cells International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1208107
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hu, Chengyu…[et al.]. Transplantation Site Affects the Outcomes of Adipose-Derived Stem Cell-Based Therapy for Retinal Degeneration. Stem Cells International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1208107
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hu, Chengyu& La, Huanzhi& Wei, Xuancheng& Zhou, Yue& Ou, Qingjian& Chen, Zhiyang…[et al.]. Transplantation Site Affects the Outcomes of Adipose-Derived Stem Cell-Based Therapy for Retinal Degeneration. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1208107
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208107
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر