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Medium Perfusion Flow Improves Osteogenic Commitment of Human Stromal Cells
المؤلفون المشاركون
Giordano, Emanuele
Pasini, Alice
Lovecchio, Joseph
Ferretti, Giulia
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-05-02
دولة النشر
مصر
عدد الصفحات
10
الملخص EN
Dynamic culture protocols have recently emerged as part of (bone) tissue engineering strategies due to their ability to represent a more physiological cell environment in vitro.
Here, we described how a perfusion flow induced by a simple bioreactor system improves proliferation and osteogenic commitment of human bone marrow stromal cells.
L88/5 cells were cultured in poly(methyl methacrylate) custom-milled communicating well plates, in the presence of an osteogenic cocktail containing 1α,25-dihydroxyvitamin D3, L-ascorbic acid 2-phosphate, and β-glycerophosphate.
The dynamic cell culture was maintained under perfusion flow stimulation at 1 mL/min for up to 4 days and compared with a static control condition.
A cell viability assay showed that the proliferation associated with the dynamic cell culture was 20% higher vs.
the static condition.
A significantly higher upregulation of the osteogenic markers runt-related transcription factor 2 (RUNX2), collagen type I (COL1A1), osteocalcin (BGLAP), alkaline phosphatase (ALPL), and osteopontin (SPP1) was detected when the perfusion flow stimulation was administered to the cells treated with the osteogenic cocktail.
An in silico analysis showed that in the dynamic cell culture condition (i) the shear stress in the proximity of the cell layer approximates 10-3 Pa, (ii) the nutrient and the waste product concentration is more homogeneously distributed than in the static counterpart, and (iii) perfusion flow was associated with higher nutrient consumption.
In summary, increased cell proliferation and enhanced early phenotype commitment indicate that dynamic cell culture conditions, delivered via bioreactor systems, produce an enhanced in vitro environment for both basic and translational research in tissue engineering and regenerative medicine.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pasini, Alice& Lovecchio, Joseph& Ferretti, Giulia& Giordano, Emanuele. 2019. Medium Perfusion Flow Improves Osteogenic Commitment of Human Stromal Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1208165
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pasini, Alice…[et al.]. Medium Perfusion Flow Improves Osteogenic Commitment of Human Stromal Cells. Stem Cells International No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1208165
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pasini, Alice& Lovecchio, Joseph& Ferretti, Giulia& Giordano, Emanuele. Medium Perfusion Flow Improves Osteogenic Commitment of Human Stromal Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1208165
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208165
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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