Adipose-Derived Stem Cells from Fat Tissue of Breast Cancer Microenvironment Present Altered Adipogenic Differentiation Capabilities
المؤلفون المشاركون
Ghilardi, Giorgio
Lesma, Elena
Vasco, C.
Ciusani, E.
Rey, Federica
Massihnia, Daniela
Nava, Sara
Al Haj, Ghina
Carelli, Stephana
Di Giulio, Anna Maria
Gorio, Alfredo
Opocher, Enrico
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-08-14
دولة النشر
مصر
عدد الصفحات
15
الملخص EN
Mesenchymal stem cells (MSCs) are multipotent cells able to differentiate into multiple cell types, including adipocytes, osteoblasts, and chondrocytes.
The role of adipose-derived stem cells (ADSCs) in cancers is significantly relevant.
They seem to be involved in the promotion of tumour development and progression and relapse processes.
For this reason, investigating the effects of breast cancer microenvironment on ADSCs is of high importance in order to understand the relationship between tumour cells and the surrounding stromal cells.
With the current study, we aimed to investigate the specific characteristics of human ADSCs isolated from the adipose tissue of breast tumour patients.
We compared ADSCs obtained from periumbilical fat (PF) of controls with ADSCs obtained from adipose tissue of breast cancer- (BC-) bearing patients.
We analysed the surface antigens and the adipogenic differentiation ability of both ADSC populations.
C/EBPδ expression was increased in PF and BC ADSCs induced to differentiate compared to the control while PPARγ and FABP4 expressions were enhanced only in PF ADSCs.
Conversely, adiponectin expression was reduced in PF-differentiated ADSCs while it was slightly increased in differentiated BC ADSCs.
By means of Oil Red O staining, we further observed an impaired differentiation capability of BC ADSCs.
To investigate this aspect more in depth, we evaluated the effect of selective PPARγ activation and nutritional supplementation on the differentiation efficiency of BC ADSCs, noting that it was only with a strong differentiation stimuli that the process took place.
Furthermore, we observed no response in BC ADSCs to the PPARγ inhibitor T0070907, showing an impaired activation of this receptor in adipose cells surrounding the breast cancer microenvironment.
In conclusion, our study shows an impaired adipogenic differentiation capability in BC ADSCs.
This suggests that the tumour microenvironment plays a key role in the modulation of the adipose microenvironment located in the surrounding tissue.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rey, Federica& Lesma, Elena& Massihnia, Daniela& Ciusani, E.& Nava, Sara& Vasco, C.…[et al.]. 2019. Adipose-Derived Stem Cells from Fat Tissue of Breast Cancer Microenvironment Present Altered Adipogenic Differentiation Capabilities. Stem Cells International،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1208212
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rey, Federica…[et al.]. Adipose-Derived Stem Cells from Fat Tissue of Breast Cancer Microenvironment Present Altered Adipogenic Differentiation Capabilities. Stem Cells International No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1208212
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rey, Federica& Lesma, Elena& Massihnia, Daniela& Ciusani, E.& Nava, Sara& Vasco, C.…[et al.]. Adipose-Derived Stem Cells from Fat Tissue of Breast Cancer Microenvironment Present Altered Adipogenic Differentiation Capabilities. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1208212
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208212
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر