Bone Marrow-Derived CD44+ Cells Migrate to Tissue-Engineered Constructs via SDF-1CXCR4-JNK Pathway and Aid Bone Repair
المؤلفون المشاركون
Dong, Shiwu
Hou, Tianyong
Lu, Yanzhu
Xing, Junchao
Yin, Xiaolong
Zhu, Xiaobo
Yang, Aijun
Luo, Jiyue
Gou, Jing
Xu, Jianzhong
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-07-24
دولة النشر
مصر
عدد الصفحات
14
الملخص EN
Background and Aims.
Host-derived cells play crucial roles in the regeneration process of tissue-engineered constructs (TECs) during the treatment of large segmental bone defects (LSBDs).
However, their identity, source, and cell recruitment mechanisms remain elusive.
Methods.
A complex model was created using mice by combining methods of GFP+ bone marrow transplantation (GFP-BMT), parabiosis (GFP+-BMT and wild-type mice), and femoral LSBD, followed by implantation of TECs or DBM scaffolds.
Postoperatively, the migration of host BM cells was detected by animal imaging and immunofluorescent staining.
Bone repair was evaluated by micro-CT.
Signaling pathway repressors including AMD3100 and SP600125 associated with the migration of BM CD44+ cells were further investigated.
In vitro, transwell migration and western-blotting assays were performed to verify the related signaling pathway.
In vivo, the importance of the SDF-1/CXCR4-JNK pathway was validated by ELISA, fluorescence-activated cell sorting (FACS), immunofluorescent staining, and RT-PCR.
Results.
First, we found that host cells recruited to facilitate TEC-mediated bone repair were derived from bone marrow and most of them express CD44, indicating the significance of CD44 in the migration of bone marrow cells towards donor MSCs.
Then, the predominant roles of SDF-1/CXCR4 and downstream JNK in the migration of BM CD44+ cells towards TECs were demonstrated.
Conclusion.
Together, we demonstrated that during bone repair promoted by TECs, BM-derived CD44+ cells were essential and their migration towards TECs could be regulated by the SDF-1/CXCR4-JNK signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lu, Yanzhu& Xing, Junchao& Yin, Xiaolong& Zhu, Xiaobo& Yang, Aijun& Luo, Jiyue…[et al.]. 2019. Bone Marrow-Derived CD44+ Cells Migrate to Tissue-Engineered Constructs via SDF-1CXCR4-JNK Pathway and Aid Bone Repair. Stem Cells International،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1208234
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lu, Yanzhu…[et al.]. Bone Marrow-Derived CD44+ Cells Migrate to Tissue-Engineered Constructs via SDF-1CXCR4-JNK Pathway and Aid Bone Repair. Stem Cells International No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1208234
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lu, Yanzhu& Xing, Junchao& Yin, Xiaolong& Zhu, Xiaobo& Yang, Aijun& Luo, Jiyue…[et al.]. Bone Marrow-Derived CD44+ Cells Migrate to Tissue-Engineered Constructs via SDF-1CXCR4-JNK Pathway and Aid Bone Repair. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1208234
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208234
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر