A Comparison of Four Molecular Methods for Detection of Aflatoxin-Producing Aspergillus in Peanut and Dried Shrimp Samples Collected from Local Markets around Pathum Thani Province, Thailand
المؤلفون المشاركون
Kanchanaphum, Panan
Kumsiri, Ratchanok
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-24
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Aspergillus flavus is an aflatoxin-producing fungus which is poisonous to humans and animals when consumed.
Detecting the fungus can help to prevent this danger.
The four molecular methods, namely, conventional isothermal amplification (LAMP), PCR, quantitative LAMP (qLAMP), and qPCR, were compared to determine their efficiency for A.
flavus detection.
Thirty samples of peanut and dried shrimp were collected from 15 markets around Pathum Thani Province in Thailand.
The samples were artificially infected with 108 conidia/ml of A.
flavus for 1 hr and enriched for one day to represent real contamination.
The results show that the sensitivity detection for A.
flavus in PCR, LAMP, qPCR, and qLAMP was 50 ng, 5 ng, 5 pg, and 5 pg, respectively.
Aspergillus in 30 peanut and dried shrimp from the market was detected by all four methods.
The detection rate was about 20%, 60%, 100%, and 100% with PCR, LAMP, qPCR, and qLAMP, respectively.
The molecular detection technique, especially LAMP, qPCR, and qLAMP, can detect this pathogenic fungi very rapidly with high sensitivity and reliability in comparison to conventional PCR.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kumsiri, Ratchanok& Kanchanaphum, Panan. 2020. A Comparison of Four Molecular Methods for Detection of Aflatoxin-Producing Aspergillus in Peanut and Dried Shrimp Samples Collected from Local Markets around Pathum Thani Province, Thailand. Scientifica،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1208257
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kumsiri, Ratchanok& Kanchanaphum, Panan. A Comparison of Four Molecular Methods for Detection of Aflatoxin-Producing Aspergillus in Peanut and Dried Shrimp Samples Collected from Local Markets around Pathum Thani Province, Thailand. Scientifica No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1208257
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kumsiri, Ratchanok& Kanchanaphum, Panan. A Comparison of Four Molecular Methods for Detection of Aflatoxin-Producing Aspergillus in Peanut and Dried Shrimp Samples Collected from Local Markets around Pathum Thani Province, Thailand. Scientifica. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1208257
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208257
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر