Increased Neuronal Differentiation Efficiency in High Cell Density-Derived Induced Pluripotent Stem Cells
المؤلفون المشاركون
Hermann, Andreas
Srimasorn, Sumitra
Storch, Alexander
Kirsch, Matthias
Hallmeyer-Ellgner, Susanne
Lindemann, Dirk
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-04
دولة النشر
مصر
عدد الصفحات
8
الملخص EN
Human pluripotent stem cells (hPSCs), including induced pluripotent stem cells (iPSCs), provide access to hard-to-obtain cells for studies under physiological and disease conditions.
For the study of neurodegenerative diseases, especially sporadic cases where the “disease condition” might be restricted towards the neuroectodermal lineage, obtaining the affected neurons is important to help unravel the underlying molecular mechanism leading to the diseases.
Although differentiation of iPSCs to neural lineage allows acquisition of cell types of interest, the technology suffers from low efficiency leading to low yield of neurons.
Here, we investigated the potential of adult neuroprogenitor cells (aNPCs) for iPSC derivation and possible confounders such as cell density of infected NPCs on their subsequent neuronal differentiation potential from reprogrammed cells under isogenic conditions.
Characterized hiPSCs of defined cell densities generated from aNPCs were subjected to neuronal differentiation on PA6 stromal cells.
The results showed that hiPSC clones obtained from low seeding density (iPSC-aNPCLow) differentiated less efficiently compared to those from higher density (iPSC-aNPCHigh).
Our findings might help to further improve the yield and quality of neurons for in vitro modelling of neurodegenerative diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Srimasorn, Sumitra& Kirsch, Matthias& Hallmeyer-Ellgner, Susanne& Lindemann, Dirk& Storch, Alexander& Hermann, Andreas. 2019. Increased Neuronal Differentiation Efficiency in High Cell Density-Derived Induced Pluripotent Stem Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1208339
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Srimasorn, Sumitra…[et al.]. Increased Neuronal Differentiation Efficiency in High Cell Density-Derived Induced Pluripotent Stem Cells. Stem Cells International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1208339
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Srimasorn, Sumitra& Kirsch, Matthias& Hallmeyer-Ellgner, Susanne& Lindemann, Dirk& Storch, Alexander& Hermann, Andreas. Increased Neuronal Differentiation Efficiency in High Cell Density-Derived Induced Pluripotent Stem Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1208339
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208339
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر