Genomics Analysis of Metabolic Pathways of Human Stem Cell-Derived Microglia-Like Cells and the Integrated Cortical Spheroids
المؤلفون المشاركون
Sart, Sébastien
Song, Liqing
Li, Yan
Bejoy, Julie
Yuan, Xuegang
Hua, Thien
Jeske, Richard
Sang, Qing-Xiang Amy
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-21، 21ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-18
دولة النشر
مصر
عدد الصفحات
21
الملخص EN
Brain spheroids or organoids derived from human pluripotent stem cells (hiPSCs) are still not capable of completely recapitulating in vivo human brain tissue, and one of the limitations is lack of microglia.
To add built-in immune function, coculture of the dorsal forebrain spheroids with isogenic microglia-like cells (D-MG) was performed in our study.
The three-dimensional D-MG spheroids were analyzed for their transcriptome and compared with isogenic microglia-like cells (MG).
Cortical spheroids containing microglia-like cells displayed different metabolic programming, which may affect the associated phenotype.
The expression of genes related to glycolysis and hypoxia signaling was increased in cocultured D-MG spheroids, indicating the metabolic shift to aerobic glycolysis, which is in favor of M1 polarization of microglia-like cells.
In addition, the metabolic pathways and the signaling pathways involved in cell proliferation, cell death, PIK3/AKT/mTOR signaling, eukaryotic initiation factor 2 pathway, and Wnt and Notch pathways were analyzed.
The results demonstrate the activation of mTOR and p53 signaling, increased expression of Notch ligands, and the repression of NF-κB and canonical Wnt pathways, as well as the lower expression of cell cycle genes in the cocultured D-MG spheroids.
This analysis indicates that physiological 3-D microenvironment may reshape the immunity of in vitro cortical spheroids and better recapitulate in vivo brain tissue function for disease modeling and drug screening.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bejoy, Julie& Yuan, Xuegang& Song, Liqing& Hua, Thien& Jeske, Richard& Sart, Sébastien…[et al.]. 2019. Genomics Analysis of Metabolic Pathways of Human Stem Cell-Derived Microglia-Like Cells and the Integrated Cortical Spheroids. Stem Cells International،Vol. 2019, no. 2019, pp.1-21.
https://search.emarefa.net/detail/BIM-1208442
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bejoy, Julie…[et al.]. Genomics Analysis of Metabolic Pathways of Human Stem Cell-Derived Microglia-Like Cells and the Integrated Cortical Spheroids. Stem Cells International No. 2019 (2019), pp.1-21.
https://search.emarefa.net/detail/BIM-1208442
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bejoy, Julie& Yuan, Xuegang& Song, Liqing& Hua, Thien& Jeske, Richard& Sart, Sébastien…[et al.]. Genomics Analysis of Metabolic Pathways of Human Stem Cell-Derived Microglia-Like Cells and the Integrated Cortical Spheroids. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-21.
https://search.emarefa.net/detail/BIM-1208442
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208442
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر