Prx1-Expressing Progenitor Primary Cilia Mediate Bone Formation in response to Mechanical Loading in Mice
المؤلفون المشاركون
Moore, Emily R.
Chen, Julia C.
Jacobs, Christopher R.
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-11
دولة النشر
مصر
عدد الصفحات
9
الملخص EN
Increases in mechanical loading can enhance the addition of new bone, altering geometry and density such that bones better withstand higher forces.
Bone-forming osteoblasts have long been thought to originate from progenitors, but the exact source is yet to be identified.
Previous studies indicate osteogenic precursors arise from Prx1-expressing progenitors during embryonic development and adult fracture repair.
However, it is unknown whether this cell population is also a source for mechanically induced active osteoblasts.
We first identified that Prx1 is expressed in skeletally mature mouse periosteum, a thin tissue covering the surface of the bone that is rich in osteoprogenitors.
We then traced Prx1 progenitor lineage using a transgenic mouse model carrying both a Prx1-driven tamoxifen-inducible Cre and a ROSA-driven lacZ reporter gene.
Cells that expressed Prx1 when compressive axial loading was applied were detected within the cortical bone days after stimulation, indicating osteocytes are of Prx1-expressing cell origin.
In addition, we evaluated how these cells sense and respond to physical stimulation in vivo by disrupting their primary cilia, which are antenna-like sensory organelles known to enhance mechanical and chemical signaling kinetics.
Although Prx1-driven primary cilium disruption did not affect osteoblast recruitment to the bone surface, the relative mineral apposition and bone formation rates were decreased by 53% and 34%, respectively.
Thus, this cell population contributes to load-induced bone formation, and primary cilia are needed for a complete response.
Interestingly, Prx1-expressing progenitors are easily extracted from periosteum and are perhaps an attractive alternative to marrow stem cells for bone tissue regeneration strategies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Moore, Emily R.& Chen, Julia C.& Jacobs, Christopher R.. 2019. Prx1-Expressing Progenitor Primary Cilia Mediate Bone Formation in response to Mechanical Loading in Mice. Stem Cells International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1208600
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Moore, Emily R.…[et al.]. Prx1-Expressing Progenitor Primary Cilia Mediate Bone Formation in response to Mechanical Loading in Mice. Stem Cells International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1208600
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Moore, Emily R.& Chen, Julia C.& Jacobs, Christopher R.. Prx1-Expressing Progenitor Primary Cilia Mediate Bone Formation in response to Mechanical Loading in Mice. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1208600
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208600
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر