Characterization of the Angiogenic Potential of Human Regulatory Macrophages (Mreg)‎ after IschemiaReperfusion Injury In Vitro

المؤلفون المشاركون

Fändrich, Fred
Cremer, Jochen
Hummitzsch, Lars
Zitta, Karina
Rusch, Rene
Gross, Justus
Berndt, Rouven
Albrecht, Martin
Steinfath, Markus

المصدر

Stem Cells International

العدد

المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2019-06-25

دولة النشر

مصر

عدد الصفحات

10

الملخص EN

Ischemia/reperfusion- (I/R-) induced organ damage represents one of the main causes of death worldwide, and new strategies to reduce I/R injury are urgently needed.

We have shown that programmable cells of monocytic origin (PCMO) respond to I/R with the release of angiogenic mediators and that transplantation of PCMO results in increased neovascularization.

Human regulatory macrophages (Mreg), which are also of monocytic origin, have been successfully employed in clinical transplantation studies due to their immunomodulatory properties.

Here, we investigated whether Mreg also possess angiogenic potential in vitro and could represent a treatment option for I/R-associated illnesses.

Mreg were differentiated using peripheral blood monocytes from different donors (N=14) by incubation with M-CSF and human AB serum and stimulation with INF-gamma.

Mreg cultures were subjected to 3 h of hypoxia and 24 h of reoxygenation (resembling I/R) or the respective nonischemic control.

Cellular resilience, expression of pluripotency markers, secretion of angiogenic proteins, and influence on endothelial tube formation as a surrogate marker for angiogenesis were investigated.

Mreg showed resilience against I/R that did not lead to increased cell damage.

Mreg express DHRS9 as well as IDO and display a moderate to low expression pattern of several pluripotency genes (e.g., NANOG, OCT-4, and SOX2).

I/R resulted in an upregulation of IDO (p<0.001) while C-MYC and KLF4 were downregulated (p<0.001 and p<0.05).

Proteome profiling revealed the secretion of numerous angiogenic proteins by Mreg of which several were strongly upregulated by I/R (e.g., MIP-1alpha, 19.9-fold; GM-CSF, 19.2-fold; PTX3, 5.8-fold; IL-1β, 5.2-fold; and MCP-1, 4.7-fold).

The angiogenic potential of supernatants from Mreg subjected to I/R remains inconclusive.

While Mreg supernatants from 3 donors induced tube formation, 2 supernatants were not effective.

We suggest that Mreg may prove beneficial as a cell therapy-based treatment option for I/R-associated illnesses.

However, donor characteristics seem to crucially influence the effectiveness of Mreg treatment.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Hummitzsch, Lars& Zitta, Karina& Rusch, Rene& Cremer, Jochen& Steinfath, Markus& Gross, Justus…[et al.]. 2019. Characterization of the Angiogenic Potential of Human Regulatory Macrophages (Mreg) after IschemiaReperfusion Injury In Vitro. Stem Cells International،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1208723

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Hummitzsch, Lars…[et al.]. Characterization of the Angiogenic Potential of Human Regulatory Macrophages (Mreg) after IschemiaReperfusion Injury In Vitro. Stem Cells International No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1208723

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Hummitzsch, Lars& Zitta, Karina& Rusch, Rene& Cremer, Jochen& Steinfath, Markus& Gross, Justus…[et al.]. Characterization of the Angiogenic Potential of Human Regulatory Macrophages (Mreg) after IschemiaReperfusion Injury In Vitro. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1208723

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1208723