Mesenchymal Stromal Cells from the Epidermis and Dermis of Psoriasis Patients: Morphology, Immunophenotype, Differentiation Patterns, and Regulation of T Cell Proliferation
المؤلفون المشاركون
Mayani, Hector
Monroy-García, A.
Castro-Manrreza, Marta E.
Montesinos, Juan J.
Castro-Escamilla, Octavio
Cortés-Morales, A.
Hernández-Estévez, E.
Hernández-Cristino, J.
Bonifaz, Laura
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-01
دولة النشر
مصر
عدد الصفحات
13
الملخص EN
Psoriasis is a skin disease characterized by hyperproliferation of keratinocytes and chronic inflammation.
Mesenchymal stem/stromal cells (MSCs) exhibit an immunoregulatory function that can be altered in the skin of these patients.
However, to date, the presence and functional capacity of MSCs in the dermis and epidermis of patients with psoriasis have not been fully established.
In the present study, we evaluated the presence of MSCs in the skin of patients by obtaining adherent cells from the dermis and epidermis of lesional and nonlesional areas and characterizing them in a comparative manner with corresponding cells obtained from the dermis (HD-MSCs) and epidermis (HE-MSCs) of healthy donors.
We determined whether the adherent cells had immunophenotypic profiles and differentiation potentials that were characteristic of MSCs.
In addition, we analyzed their immunosuppression function by evaluating their capacity to decrease T cell proliferation.
Our results indicate the presence of MSCs in the dermis and epidermis of healthy donors and patients with psoriasis; adherent cells from all skin sources exhibited MSC characteristics, such as expression of CD73, CD90, and CD105 markers and a lack of hematopoietic and endothelial marker expression.
However, the cell populations obtained showed differences in differentiation potential toward adipogenic, osteogenic, and chondrogenic lineages.
In addition, we observed a low MSC obtention frequency in nonlesional epidermal samples (NLE-MSCs), which also showed alterations in morphology and proliferation rate.
Interestingly, MSCs from both the nonlesional dermis (NLD-MSCs) and lesional dermis (LD-MSCs) showed higher HLA class I antigen (HLA-I) expression than HD-MSCs.
Moreover, NLD-MSCs showed a low T cell proliferation suppression capacity.
In summary, this study demonstrates the presence of MSCs in the epidermis and dermis of patients with psoriasis and suggests that such cells may favor the inflammatory process and thus psoriatic lesion development through high HLA-I expression and low immunosuppression capacity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Castro-Manrreza, Marta E.& Bonifaz, Laura& Castro-Escamilla, Octavio& Monroy-García, A.& Cortés-Morales, A.& Hernández-Estévez, E.…[et al.]. 2019. Mesenchymal Stromal Cells from the Epidermis and Dermis of Psoriasis Patients: Morphology, Immunophenotype, Differentiation Patterns, and Regulation of T Cell Proliferation. Stem Cells International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1208851
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Castro-Manrreza, Marta E.…[et al.]. Mesenchymal Stromal Cells from the Epidermis and Dermis of Psoriasis Patients: Morphology, Immunophenotype, Differentiation Patterns, and Regulation of T Cell Proliferation. Stem Cells International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1208851
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Castro-Manrreza, Marta E.& Bonifaz, Laura& Castro-Escamilla, Octavio& Monroy-García, A.& Cortés-Morales, A.& Hernández-Estévez, E.…[et al.]. Mesenchymal Stromal Cells from the Epidermis and Dermis of Psoriasis Patients: Morphology, Immunophenotype, Differentiation Patterns, and Regulation of T Cell Proliferation. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1208851
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208851
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر