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The Conditioned Medium of Calcined Tooth Powder Promotes the Osteogenic and Odontogenic Differentiation of Human Dental Pulp Stem Cells via MAPK Signaling Pathways
المؤلفون المشاركون
Yu, Jinhua
Wang, Yanqiu
Wu, Jintao
Li, Zehan
Gobin, Romila
Li, Na
Fan, Yuan
Gu, Yongchun
Pan, Yin
Zhou, Zuomin
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-03-19
دولة النشر
مصر
عدد الصفحات
13
الملخص EN
The calcined tooth powder (CTP), a type of allogeneic biomimetic mineralized material, has been confirmed that can promote new bone formation when obtained at high temperature.
The aim of this study was to investigate effects of the conditioned medium of calcined tooth powder (CTP-CM) on the osteogenic and odontogenic differentiation of human dental pulp stem cells (hDPSCs) and the underlying mechanisms involved.
First, ALP activity assay determined that 200 μg/mL was the optimal concentration of CTP-CM for the following experiments.
CTP-CM had no significant effect on the proliferation of hDPSCs as indicated by CCK-8 and FCM analysis.
Both the gene and protein (DSPP/DSPP, RUNX2/RUNX2, OCN/OCN, OSX/OSX, OPN/OPN, ALP/ALP, and COL-1/COL-1) expression levels increased in the CTP-CM-induced hDPSC group as compared with those in the control group at day 3 or 7, showing the positive regulation of CTP-CM on the osteo/odontogenic differentiation of hDPSCs.
Mechanistically, MAPK signaling pathways were activated after the CTP-CM treatment, and the inhibitors targeting MAPK were identified which weakened the effects of CTM-CM on the committed differentiation of hDPSCs.
These findings could lead to the creation of stem cell therapies for dental regeneration.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wu, Jintao& Li, Na& Fan, Yuan& Wang, Yanqiu& Gu, Yongchun& Li, Zehan…[et al.]. 2019. The Conditioned Medium of Calcined Tooth Powder Promotes the Osteogenic and Odontogenic Differentiation of Human Dental Pulp Stem Cells via MAPK Signaling Pathways. Stem Cells International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1208891
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wu, Jintao…[et al.]. The Conditioned Medium of Calcined Tooth Powder Promotes the Osteogenic and Odontogenic Differentiation of Human Dental Pulp Stem Cells via MAPK Signaling Pathways. Stem Cells International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1208891
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wu, Jintao& Li, Na& Fan, Yuan& Wang, Yanqiu& Gu, Yongchun& Li, Zehan…[et al.]. The Conditioned Medium of Calcined Tooth Powder Promotes the Osteogenic and Odontogenic Differentiation of Human Dental Pulp Stem Cells via MAPK Signaling Pathways. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1208891
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208891
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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