Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells

المؤلفون المشاركون

Winston, Tackla S.
Suddhapas, Kantaphon
Wang, Chenyan
Ramos, Rafael
Soman, Pranav
Ma, Zhen

المصدر

Stem Cells International

العدد

المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2019-01-15

دولة النشر

مصر

عدد الصفحات

11

الملخص EN

Combination of stem cell technology and 3D biofabrication approaches provides physiological similarity to in vivo tissues and the capability of repairing and regenerating damaged human tissues.

Mesenchymal stem cells (MSCs) have been widely used for regenerative medicine applications because of their immunosuppressive properties and multipotent potentials.

To obtain large amount of high-quality MSCs without patient donation and invasive procedures, we differentiated MSCs from human-induced pluripotent stem cells (hiPSC-MSCs) using serum-free E6 media supplemented with only one growth factor (bFGF) and two small molecules (SB431542 and CHIR99021).

The differentiated cells showed a high expression of common MSC-specific surface markers (CD90, CD73, CD105, CD106, CD146, and CD166) and a high potency for osteogenic and chondrogenic differentiation.

With these cells, we have been able to manufacture MSC tissue rings with high consistency and robustness in pluronic-coated reusable PDMS devices.

The MSC tissue rings were characterized based on inner diameter and outer ring diameter and observed cell-type-dependent tissue contraction induced by cell-matrix interaction.

Our approach of simplified hiPSC-MSC differentiation, modular fabrication procedure, and serum-free culture conditions has a great potential for scalable manufacturing of MSC tissue rings for different regenerative medicine applications.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Winston, Tackla S.& Suddhapas, Kantaphon& Wang, Chenyan& Ramos, Rafael& Soman, Pranav& Ma, Zhen. 2019. Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1209099

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Winston, Tackla S.…[et al.]. Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells. Stem Cells International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1209099

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Winston, Tackla S.& Suddhapas, Kantaphon& Wang, Chenyan& Ramos, Rafael& Soman, Pranav& Ma, Zhen. Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1209099

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1209099