The DNMT1miR-34a Axis Is Involved in the Stemness of Human Osteosarcoma Cells and Derived Stem-Like Cells
المؤلفون المشاركون
Liang, Xiao
Xu, Chang
Wang, Wanchun
Li, Xiang
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-20، 20ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-10-31
دولة النشر
مصر
عدد الصفحات
20
الملخص EN
The DNA methyltransferase 1 (DNMT1)/miR-34a axis promoted carcinogenesis of various types of cancers.
However, no literature reported its contribution to the stemness of osteosarcoma cancer stem-like cells (OSLCs).
We sought to determine whether the DNMT1/miR-34a axis facilitates the stemness of OSLCs.
We here revealed the higher DNMT1 activity and expression, lower miR-34a expression with high methylation of its promoter, and stronger stemness of OSLCs, as manifested by elevated sphere and colony formation capacities; CD133, CD44, ABCG2, Bmi1, Sox2, and Oct4 protein amounts in vitro; and carcinogenicity in a nude mouse xenograft model, when compared to the parental U2OS cells.
5-Azacytidine (Aza-dC) repressed DNMT1 activation and upregulated miR-34a expression by promoter demethylation and suppressed the stemness of OSLCs in a dose-dependent manner.
DNMT1 knockdown increased miR-34a and reduced the stemness of OSLCs.
Transfection with a miR-34a mimic repressed the stemness of OSLCs but did not alter DNMT1 activity and expression.
Conversely, DNMT1 overexpression declined miR-34a levels, promoting the stemness of U2OS cells.
Transfection with a miR-34a inhibitor enhanced the stemness of U2OS cells, without affecting the DNMT1 activity and expression.
Importantly, reexpression of miR-34a could rescue the effects of DNMT1 overexpression on miR-34a inhibition as well as the stemness promotion without affecting the activity and expression of DNMT1.
Our results revealed that aberrant activation of DNMT1 caused promoter methylation of miR-34a, leading to miR-34a underexpression, and the role of the DNMT1/miR-34a axis in promoting and sustaining the stemness of OSLCs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liang, Xiao& Xu, Chang& Wang, Wanchun& Li, Xiang. 2019. The DNMT1miR-34a Axis Is Involved in the Stemness of Human Osteosarcoma Cells and Derived Stem-Like Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-20.
https://search.emarefa.net/detail/BIM-1209431
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liang, Xiao…[et al.]. The DNMT1miR-34a Axis Is Involved in the Stemness of Human Osteosarcoma Cells and Derived Stem-Like Cells. Stem Cells International No. 2019 (2019), pp.1-20.
https://search.emarefa.net/detail/BIM-1209431
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liang, Xiao& Xu, Chang& Wang, Wanchun& Li, Xiang. The DNMT1miR-34a Axis Is Involved in the Stemness of Human Osteosarcoma Cells and Derived Stem-Like Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-20.
https://search.emarefa.net/detail/BIM-1209431
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1209431
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر